New polymorphic mitochondrial markers for sponge phylogeography

Phylogeography and population genetic studies in the Porifera have been limited by the lack of available polymorphic DNA markers. In this paper, we tested four new mitochondrial markers in nine demosponge species from a wide taxonomic range: partial sequences of the ATP synthase 6 (ATP6) and the cyt...

Full description

Saved in:
Bibliographic Details
Published in:Journal of the Marine Biological Association of the United Kingdom 2011-08, Vol.91 (5), p.1015-1022
Main Authors: Rua, Cintia P.J., Zilberberg, Carla, Solé-Cava, Antonio M.
Format: Article
Language:English
Subjects:
Animal and plant ecology
Animal, plant and microbial ecology
Animals
Autoecology
Bats
Biological and medical sciences
Cytochrome
Fundamental and applied biological sciences. Psychology
Genes
Haplotypes
Invertebrates
Mammalia
Marine
Mitochondrial DNA
Phylogeography
Population genetics
Porifera
Taxonomy
Vertebrata
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Phylogeography and population genetic studies in the Porifera have been limited by the lack of available polymorphic DNA markers. In this paper, we tested four new mitochondrial markers in nine demosponge species from a wide taxonomic range: partial sequences of the ATP synthase 6 (ATP6) and the cytochrome oxidase 2 (CO2) genes and two spacers: one located between ATP6 and CO2 and the other between the NADH dehydrogenase subunit 5 (ND5) and the small subunit ribosomal RNA (rns) genes. The new markers presented levels of nucleotide diversity up to 2.4 times higher (π = 0.015 for CO2) than those observed for the most commonly used mitochondrial marker in sponges, the cytochrome oxidase 1 gene (π = 0.006), making them suitable for alpha-level systematics, phylogeography and population genetics studies. 
ISSN:0025-3154
1469-7769
DOI:10.1017/S0025315410002122