Technical note: Development of an enzyme-linked immunosorbent assay for the determination of florfenicol and thiamphenicol in swine feed

One polyclonal antibody against florfenicol and thiamphenicol was produced and a competitive ELISA was developed for the detection of florfenicol and thiamphenicol in swine feed. The ELISA gave a 50% inhibiting concentration of 1.02 ng/mL for florfenicol. For swine feed fortified with 0.05 to 3.0 mg...

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Bibliographic Details
Published in:Journal of animal science 2011-11, Vol.89 (11), p.3612-3616
Main Authors: Luo, P. J, Jiang, W. X, Chen, X, Shen, J. Z, Wu, Y. N
Format: Article
Language:English
Subjects:
Animal Feed - analysis
Animal productions
Animals
Anti-Bacterial Agents - analysis
Biological and medical sciences
enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
Enzyme-Linked Immunosorbent Assay - veterinary
Feed and pet food industries
florfenicol
Food industries
fortified feed
Fundamental and applied biological sciences. Psychology
mass spectrometry
screening
Swine
Terrestrial animal productions
Thiamphenicol - analogs & derivatives
Thiamphenicol - analysis
Vertebrates
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Summary:One polyclonal antibody against florfenicol and thiamphenicol was produced and a competitive ELISA was developed for the detection of florfenicol and thiamphenicol in swine feed. The ELISA gave a 50% inhibiting concentration of 1.02 ng/mL for florfenicol. For swine feed fortified with 0.05 to 3.0 mg/kg, the interassay recoveries of florfenicol and thiamphenicol ranged from 86.4 to 118.6%, whereas intraassay recoveries of both drug ranged from 90.1 to 126.5% with less than 15% CV. Results obtained from HPLC-tandem mass spectrometry indicated this ELISA procedure could be used as a convenient method for rapid screening of florfenicol and thiamphenicol in swine feed.
ISSN:0021-8812
1525-3163
DOI:10.2527/jas.2010-3403