Electrochemical Assay to Quantify the Hydroxyl Radical Scavenging Activity of Medicinal Plant Extracts

A new electrochemical assay has been developed which is based on quantifying the attack of OH. radicals on a self‐assembled thiol monolayer on electrodes in the presence and absence of antioxidants. The OH. radicals were produced in Fenton solutions. The radical scavengers diminish the concentration...

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Bibliographic Details
Published in:Electroanalysis (New York, N.Y.) N.Y.), 2010-02, Vol.22 (4), p.406-412
Main Authors: Hilgemann, Maurício, Scholz, Fritz, Kahlert, Heike, de Carvalho, Leandro Machado, da Rosa, Marcelo Barcellos, Lindequist, Ulrike, Wurster, Martina, do Nascimento, Paulo C., Bohrer, Denise
Format: Article
Language:English
Subjects:
Assaying
Electrochemical method
Electrodes
Extraction plants
Fenton solution
Hydroxyl radical
Hydroxyl radicals
Plant extracts
Radicals
Scavengers
Scavenging
Scavenging activity
Thiols
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Summary:A new electrochemical assay has been developed which is based on quantifying the attack of OH. radicals on a self‐assembled thiol monolayer on electrodes in the presence and absence of antioxidants. The OH. radicals were produced in Fenton solutions. The radical scavengers diminish the concentration of the OH. radicals in the Fenton solution, and the extent of thiol layer destruction is monitored with the help of a redox probe in solution, the signal of which is only generated at SAM‐free electrode surface. The results of the electrochemical assay were compared with those of the standard DPPH. assay. In case of each plant extract, an excellent linear correlation was observed between the radical scavenging activity and the concentration of plant extracts; however, the scavenging properties of different plants strongly differ in the electrochemical and the DPPH. assay. Since the proposed new method probes the reactivity of radical scavengers towards OH., one of the most important reactive oxygen radicals in tissues, it is supposed to provide much more relevant information than the DPPH. or other assays.
ISSN:1040-0397
1521-4109
1521-4109
DOI:10.1002/elan.200900385