Comparison of screening methods for antibiotics in beef kidney juice and serum

Rapid screening tests can be used as part of an efficient program designed to monitor veterinary drug residues in cattle. In this work, three rapid tests designed to screen samples for the presence of antibiotic residues, the Fast Antimicrobial Screen Test (FAST), Premi ® and Kidney Inhibition Swab...

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Bibliographic Details
Published in:Analytica chimica acta 2009-04, Vol.637 (1), p.290-297
Main Authors: Schneider, Marilyn J., Mastovska, Katerina, Lehotay, Steven J., Lightfield, Alan R., Kinsella, Brian, Shultz, Craig E.
Format: Article
Language:English
Subjects:
Analytical chemistry
Animals
Anti-Bacterial Agents - analysis
Anti-Bacterial Agents - blood
Antibiotics
Cattle
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography, Liquid - methods
Drug Residues - analysis
Exact sciences and technology
False Negative Reactions
False Positive Reactions
Kidney - chemistry
Liquid chromatography–mass spectrometry
Microbial inhibition assays
Microbial Sensitivity Tests - methods
Other chromatographic methods
Reagent Kits, Diagnostic
Screening
Sensitivity and Specificity
Spectrometric and optical methods
Tandem Mass Spectrometry - methods
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Summary:Rapid screening tests can be used as part of an efficient program designed to monitor veterinary drug residues in cattle. In this work, three rapid tests designed to screen samples for the presence of antibiotic residues, the Fast Antimicrobial Screen Test (FAST), Premi ® and Kidney Inhibition Swab (KIS™) tests, were compared using beef kidney juice and serum samples. In order to provide a realistic assessment, potentially incurred samples of beef kidney juice and serum were obtained from 235 carcasses which had been retained by inspectors in a processing plant for further testing. In addition, liquid chromatography–tandem mass spectrometry (LC–MS/MS) analysis was conducted on these samples to identify what antibiotics were present, if any, and their levels. The comparison of the three rapid screening test results with those from LC–MS/MS analysis allowed for a more complete comparison of the relative sensitivity of these analytical methods, as well as valuable information on false positive and negative response rates.
ISSN:0003-2670
1873-4324
DOI:10.1016/j.aca.2008.08.005