Bacteria attachment to surfaces – AFM force spectroscopy and physicochemical analyses

Bacterial cell was successfully coated to AFM tip (a) to measure the adhesion force between bacteria and stainless steel surfaces (b) with various surface delays. [Display omitted] ► AFM force measurement using live bacteria. ► Nature of adhesion mechanism of bacteria on stainless steel were explore...

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Bibliographic Details
Published in:Journal of colloid and interface science 2011-12, Vol.364 (1), p.213-218
Main Authors: Harimawan, Ardiyan, Rajasekar, Aruliah, Ting, Yen-Peng
Format: Article
Language:English
Subjects:
adhesion
Adhesion force
atomic force microscopy
B. subtilis
Bacillus subtilis
Bacillus subtilis - chemistry
bacterial adhesion
Biofilm
Biofilms
Chemistry
Chemistry, Physical
Exact sciences and technology
General and physical chemistry
Gram-negative bacteria
Gram-positive bacteria
Hydrophobicity
M. timonae
Massilia timonae
Microscopy, Atomic Force
Oxalobacteraceae - chemistry
P. aeruginosa
Pseudomonas aeruginosa
Pseudomonas aeruginosa - chemistry
spectroscopy
stainless steel
Stainless Steel - chemistry
Surface physical chemistry
Surface Properties
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Summary:Bacterial cell was successfully coated to AFM tip (a) to measure the adhesion force between bacteria and stainless steel surfaces (b) with various surface delays. [Display omitted] ► AFM force measurement using live bacteria. ► Nature of adhesion mechanism of bacteria on stainless steel were explored. ► Higher adhesion force on Gram-negative bacteria. ► Physicochemical analysis support force measurements. ► EPS production enhanced bacterial adhesion. Understanding bacterial adhesion to surfaces requires knowledge of the forces that govern bacterial–surface interactions. Biofilm formation on stainless steel 316 (SS316) by three bacterial species was investigated by examining surface force interaction between the cells and metal surface using atomic force microscopy (AFM). Bacterial–metal adhesion force was quantified at different surface delay time from 0 to 60 s using AFM tip coated with three different bacterial species: Gram-negative Massilia timonae and Pseudomonas aeruginosa, and Gram-positive Bacillus subtilis. The results revealed that bacterial adhesion forces on SS316 surface by Gram-negative bacteria is higher (8.53 ± 1.40 nN and 7.88 ± 0.94 nN) when compared to Gram-positive bacteria (1.44 ± 0.21 nN). Physicochemical analysis on bacterial surface properties also revealed that M. timonae and P. aeruginosa showed higher hydrophobicity and surface charges than B. subtilis along with the capability of producing extracellular polymeric substances (EPS). The higher hydrophobicity, surface charges, and greater propensity to form EPS by M. timonae and P. aeruginosa led to high adhesive force on the metal surface.
ISSN:0021-9797
1095-7103
DOI:10.1016/j.jcis.2011.08.021