T‐zone localized monocyte‐derived dendritic cells promote Th1 priming to Salmonella

Control of intracellular Salmonella infection requires Th1 priming and IFN‐γ production. Here, we show that efficient Th1 priming after Salmonella infection requires CD11c+CD11bhiF4/80+ monocyte‐derived dendritic cells (moDCs). In non‐infected spleens, moDCs are absent from T‐cell zones (T zones) of...

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Bibliographic Details
Published in:European journal of immunology 2011-09, Vol.41 (9), p.2654-2665
Main Authors: Flores‐Langarica, Adriana, Marshall, Jennifer L., Bobat, Saeeda, Mohr, Elodie, Hitchcock, Jessica, Ross, Ewan A., Coughlan, Ruth E., Khan, Mahmood, Van Rooijen, Nico, Henderson, Ian R., MacLennan, Ian C.M., Cunningham, Adam F.
Format: Article
Language:English
Subjects:
Animals
Antigen Presentation
Antigens, Differentiation - biosynthesis
APCs
CD11b Antigen - biosynthesis
CD11c Antigen - biosynthesis
Cell Communication
Cell Differentiation
Cell Proliferation
Cells, Cultured
DCs
Dendritic Cells - immunology
Dendritic Cells - metabolism
Dendritic Cells - microbiology
Dendritic Cells - pathology
Infections
Infectious diseases
Interferon-gamma - metabolism
Lymphocyte Activation
Medical research
Mice
Mice, Inbred C57BL
Monocytes - pathology
Salmonella
Salmonella - immunology
Salmonella - pathogenicity
Salmonella Infections - immunology
Spleen - pathology
T helper cell
Th1 Cells - immunology
Th1 Cells - metabolism
Th1 Cells - microbiology
Th1 Cells - pathology
Tumor Necrosis Factor-alpha - immunology
Tumor Necrosis Factor-alpha - metabolism
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Summary:Control of intracellular Salmonella infection requires Th1 priming and IFN‐γ production. Here, we show that efficient Th1 priming after Salmonella infection requires CD11c+CD11bhiF4/80+ monocyte‐derived dendritic cells (moDCs). In non‐infected spleens, moDCs are absent from T‐cell zones (T zones) of secondary lymphoid tissues, but by 24 h post‐infection moDCs are readily discernible in these sites. The accumulation of moDCs is more dependent upon bacterial viability than bacterial virulence. Kinetic studies showed that moDCs were necessary to prime but not sustain Th1 responses, while ex vivo studies showed that antigen‐experienced moDCs were sufficient to induce T‐cell proliferation and IFN‐γ production via a TNF‐α‐dependent mechanism. Importantly, moDCs and cDCs when co‐cultured induced superior Th1 differentiation than either subset alone, and this activity was independent of TNF‐α. Thus, optimal Th1 development to Salmonella requires the rapid accumulation of moDCs within T zones and their collaboration with cDCs.
ISSN:0014-2980
1521-4141
DOI:10.1002/eji.201141440