An Ion Mobility/Ion Trap/Photodissociation Instrument for Characterization of Ion Structure

A new instrument that combines ion mobility spectrometry (IMS) separations with tandem mass spectrometry (MS n ) is described. Ion fragmentation is achieved with vacuum ultraviolet photodissociation (VUV PD) and/or collision-induced dissociation (CID). The instrument is comprised of an approximately...

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Bibliographic Details
Published in:Journal of the American Society for Mass Spectrometry 2011-09, Vol.22 (9), p.1477-1485, Article s13361-011-0179-8
Main Authors: Zucker, Steven M., Lee, Sunyoung, Webber, Nathaniel, Valentine, Stephen J., Reilly, James P., Clemmer, David E.
Format: Article
Language:English
Subjects:
Analytical Chemistry
Analytical, structural and metabolic biochemistry
Bioinformatics
Biological and medical sciences
Biotechnology
Carbohydrates
Chemistry
Chemistry and Materials Science
Drift tubes
Exact sciences and technology
Fragmentation
Fragments
Fundamental and applied biological sciences. Psychology
Ionic mobility
Ions
Ions - chemistry
Isomerism
Isomers
Mass spectrometry
Miscellaneous
Models, Molecular
Organic Chemistry
Other biological molecules
Photodissociation
Proteomics
Raffinose
Raffinose - chemistry
Research Article
Scientific imaging
Spectrometric and optical methods
Spectrometry, Mass, Electrospray Ionization - instrumentation
Spectrometry, Mass, Electrospray Ionization - methods
Spectroscopy
Structural analysis
Tandem Mass Spectrometry - instrumentation
Tandem Mass Spectrometry - methods
Trisaccharides - chemistry
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Description
Summary:A new instrument that combines ion mobility spectrometry (IMS) separations with tandem mass spectrometry (MS n ) is described. Ion fragmentation is achieved with vacuum ultraviolet photodissociation (VUV PD) and/or collision-induced dissociation (CID). The instrument is comprised of an approximately 1 m long drift tube connected to a linear trap that has been interfaced to a pulsed F 2 laser (157 nm). Ion gates positioned in the front and the back of the primary drift region allow for mobility selection of specific ions prior to their storage in the ion trap, mass analysis, and fragmentation. The ion characterization advantages of the new instrument are demonstrated with the analysis of the isomeric trisaccharides, melezitose and raffinose. Mobility separation of precursor ions provides a means of separating the isomers and subsequent VUV PD generates unique fragments allowing them to be distinguished.
ISSN:1044-0305
1879-1123
DOI:10.1007/s13361-011-0179-8