Application of HR-MAS NMR spectroscopy for studying chemotype variations of Withania somnifera (L.) Dunal

Withania somnifera (L.) Dunal (Solanaceae), commonly known as Ashwagandha, is one of the most valued Indian medicinal plants with a number of pharmaceutical and nutraceutical applications. Metabolic profiling has been performed by HR‐MAS NMR spectroscopy on fresh leaf and root tissue specimens from...

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Published in:Magnetic resonance in chemistry 2011-10, Vol.49 (10), p.659-667
Main Authors: Bharti, S. K., Bhatia, Anil, Tewari, S. K., Sidhu, O. P., Roy, Raja
Format: Article
Language:English
Subjects:
HR-MAS NMR
Indian
metabolic profile
Metabolites
Methanol - chemistry
Methyl alcohol
Multivariate Analysis
NMR spectroscopy
Nuclear Magnetic Resonance, Biomolecular - methods
Plant Leaves - chemistry
Plant Leaves - metabolism
Plant Roots - chemistry
Plant Roots - metabolism
Principal component analysis
Profiling
Roots
Spectra
Withania - chemistry
Withania - metabolism
Withania somnifera
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Summary:Withania somnifera (L.) Dunal (Solanaceae), commonly known as Ashwagandha, is one of the most valued Indian medicinal plants with a number of pharmaceutical and nutraceutical applications. Metabolic profiling has been performed by HR‐MAS NMR spectroscopy on fresh leaf and root tissue specimens from four chemotypes of W. somnifera. The HR‐MAS NMR spectroscopy of lyophilized defatted leaf tissue specimens clearly distinguishes resonances of medicinally important secondary metabolites (withaferin A and withanone) and its distinctive quantitative variability among the chemotypes. A total of 41 metabolites were identified from both the leaf and root tissues of the chemotypes. The presence of methanol in leaf and root tissues of W. somnifera was detected by HR‐MAS NMR spectroscopy. Multivariate principal component analysis (PCA) on HR‐MAS 1H NMR spectra of leaves revealed clear variations in primary metabolites among the chemotypes. The results of the present study demonstrated an efficient method, which can be utilized for metabolite profiling of primary and secondary metabolites in medicinally important plants. Copyright © 2011 John Wiley & Sons, Ltd. The HR‐MAS NMR spectroscopy differentiated chemotypes of Withania somnifera (Ashwagandha) by multivariate data analysis. Results demonstrate an efficient method which can be utilized as metabolite profiling for the analysis of primary and secondary metabolites in medicinally important plants.
ISSN:0749-1581
1097-458X
1097-458X
DOI:10.1002/mrc.2817