Effects of Hormonal Supplementation on Nuclear Maturation and Cortical Granules Distribution of Canine Oocytes During Various Reproductive Stages

The aim of this study was to evaluate the effects of hCG, progesterone and oestradiol supplementation on nuclear and cytoplasmic maturation of canine oocytes cultured for 24, 48, 72 and 96 h. Oocytes obtained from 18 healthy bitches were divided into three groups according to their reproductive stat...

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Bibliographic Details
Published in:Reproduction in domestic animals 2011-10, Vol.46 (5), p.896-903
Main Authors: Apparicio, M, Alves, AE, Pires‐Butler, EA, Ribeiro, APC, Covizzi, GJ, Vicente, WRR
Format: Article
Language:English
Subjects:
anestrus
Animal reproduction
Animals
Biological and medical sciences
bitches
Cell Nucleus - physiology
chromatin
cortical granules
culture media
cytoplasm
Dogs
Dogs - physiology
estradiol
Estrous Cycle - physiology
Female
Fundamental and applied biological sciences. Psychology
germinal vesicle
Hormones
human chorionic gonadotropin
Mammalian reproduction. General aspects
meiosis
metaphase
Oocytes - physiology
plasma membrane
progesterone
Vertebrates: reproduction
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Summary:The aim of this study was to evaluate the effects of hCG, progesterone and oestradiol supplementation on nuclear and cytoplasmic maturation of canine oocytes cultured for 24, 48, 72 and 96 h. Oocytes obtained from 18 healthy bitches were divided into three groups according to their reproductive status (follicular, luteal and anoestrus stages) and cultured in TCM 199 + 25 UI/ml of hCG + 1 μg/ml of progesterone + 1 μg/ml of 17‐β oestradiol or without hormonal supplementation (control) for different periods. Then, they were stained with FITC‐LCA‐Hoescht for chromatin configuration and cortical granules distribution and evaluated under an epifluorescence microscope. Culture time and the influence of different stages of the oestrous cycle were also evaluated. The present study demonstrated that there was no significant difference among the reproductive stages. With regards to culture medium, only oocytes from the supplemented medium were able to complete meiosis; however, significant difference was only noticed in the percentage of MI stage oocytes (p 
ISSN:0936-6768
1439-0531
DOI:10.1111/j.1439-0531.2011.01761.x