Modulation of Cytokine and Chemokine Secretions in Rhesus Monkey Trophoblast Co-Culture With Decidual but not Peripheral Blood Monocyte-Derived Macrophages

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Rozner AE, Dambaeva SV, Drenzek JG, Durning M, Golos TG. Modulation of cytokine and chemokine secretions in rhesus monkey trophoblast co‐culture with decidual but not peripheral blood monocyte–derived macrophages. Am J Reprod Immunol 2011; 66: 115–127 Problem  Decidual macrophages are thou...

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Published in:American journal of reproductive immunology (1989) 2011-08, Vol.66 (2), p.115-127
Main Authors: Rozner, Ann E., Dambaeva, Svetlana V., Drenzek, Jessica G., Durning, Maureen, Golos, Thaddeus G.
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antigen processing
Cells, Cultured
Chemokine
Chemokines
Chemokines - analysis
Chemokines - immunology
Chemokines - secretion
Coculture Techniques
cytokine
Cytokines
Cytokines - analysis
Cytokines - immunology
Cytokines - secretion
decidua
Decidua - cytology
Decidua - immunology
Female
HLA-G
Leukocyte migration
Leukocytes, Mononuclear - cytology
Leukocytes, Mononuclear - immunology
Macaca mulatta
macrophage
Macrophages
Macrophages - cytology
Macrophages - immunology
Monocytes
Peripheral blood
Pregnancy
Pregnancy, Animal
Secretions
trophoblast
Trophoblasts
Trophoblasts - cytology
Trophoblasts - immunology
Trophoblasts - secretion
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Rozner AE, Dambaeva SV, Drenzek JG, Durning M, Golos TG. Modulation of cytokine and chemokine secretions in rhesus monkey trophoblast co‐culture with decidual but not peripheral blood monocyte–derived macrophages. Am J Reprod Immunol 2011; 66: 115–127 Problem  Decidual macrophages are thought to promote pregnancy success, in part through interactions with invading trophoblast cells in hemochorial placentation. However, the factors that constitute this regulatory cross talk are not well understood. Method of study  Rhesus monkey decidual and peripheral blood–derived macrophages were co‐cultured with primary Rhesus trophoblasts. Macrophage functions including cell‐surface marker expression, antigen uptake and processing, in vitro migration, and cytokine and chemokine secretions were evaluated. Results  While most macrophage functions were unchanged by trophoblast co‐culture, changes in the secretion of selected cytokines and the migration of trophoblasts were noted when decidual (but generally, not peripheral blood monocyte–derived) macrophages were cultured with trophoblasts. In addition, basal secretion differed significantly between peripheral blood–derived and decidual macrophages for a broad spectrum of cytokines. When trophoblasts were pre‐treated with an anti‐Mamu‐AG antibody, 25D3, there was no change in cytokine or chemokine secretion. Conclusion  Macrophage cytokine expression can be modulated by trophoblast co‐culture, but it remains unclear how Mamu‐AG is involved.
ISSN:1046-7408
1600-0897
DOI:10.1111/j.1600-0897.2010.00979.x