Trichinella spiralis infection rapidly induces lung inflammatory response: the lung as the site of helminthocytotoxic activity

In the present work, we studied the kinetics of the appearance of different immunological parameters in the lungs during the intestinal phase of infection with Trichinella spiralis. We also evaluated the lung's role in the retention and death of this helminth in its migratory stage. To study th...

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Bibliographic Details
Published in:Immunobiology (1979) 2011-09, Vol.216 (9), p.1054-1063
Main Authors: Gentilini, María V, Nuñez, Guillermo G, Roux, María E, Venturiello, Stella M
Format: Article
Language:English
Subjects:
Animals
Antibodies, Helminth - immunology
Cells, Cultured
Cytokines - biosynthesis
Cytokines - immunology
Cytotoxicity, Immunologic
Eosinophils - cytology
Eosinophils - immunology
Eosinophils - metabolism
Female
Immunity, Mucosal
Immunoglobulin Isotypes - biosynthesis
Immunoglobulin Isotypes - immunology
Immunohistochemistry
Inflammation - immunology
Inflammation - parasitology
Inflammation - pathology
Intestinal Mucosa - immunology
Larva - growth & development
Larva - immunology
Lung - immunology
Lung - parasitology
Lung - pathology
Mast Cells - cytology
Mast Cells - immunology
Mast Cells - metabolism
Primary Cell Culture
Rats
Rats, Wistar
Tissue Extracts
Trichinella spiralis
Trichinella spiralis - growth & development
Trichinella spiralis - immunology
Trichinellosis - immunology
Trichinellosis - parasitology
Trichinellosis - pathology
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Summary:In the present work, we studied the kinetics of the appearance of different immunological parameters in the lungs during the intestinal phase of infection with Trichinella spiralis. We also evaluated the lung's role in the retention and death of this helminth in its migratory stage. To study these parameters, we used lung extracts, lung cell suspensions and rat lung tissue sections. During the intestinal phase of infection (days 0-13 post-infection, p.i.), an inflammatory response is elicited in the lungs, which reflects humoral, cellular and functional changes. These changes included an increased number of mast cells and eosinophils and the local production of IL-4, IL-5, IL-10, TNFα, IFNγ, IL-13, CCL11 and CCL28. We found hyperplasia of the bronchus-associated lymphoid tissue (BALT). Total and specific IgA, IgE, IgG1 and IgG2a were detected locally. The retention of the migratory larvae in the lung, together with the ex vivo cytotoxic capacity of the lung cells and antibodies present in the lung extracts, suggested that the lung was one of the immune defense organs against the pathogen's migration stage.
ISSN:0171-2985
1878-3279
DOI:10.1016/j.imbio.2011.02.002