Approaches for cytogenetic and molecular analyses of small flow-sorted cell populations from childhood leukemia bone marrow samples

Discordances between minimal residual disease estimates obtained by different methods are a problem in childhood acute lymphoblastic leukemia. We aimed to optimize methods allowing the biological exploration of such discrepancies, i.e. the combination of flow-sorting of small immunophenotypically de...

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Bibliographic Details
Published in:Journal of immunological methods 2011-06, Vol.369 (1), p.69-73
Main Authors: Øbro, Nina Friesgaard, Madsen, Hans Ole, Ryder, Lars Peter, Andersen, Mette Klarskov, Schmiegelow, Kjeld, Marquart, Hanne Vibeke
Format: Article
Language:English
Subjects:
Acute lymphatic leukemia
Biological and medical sciences
Bone marrow
Bone Marrow - chemistry
Bone Marrow - immunology
Cell Separation - methods
Child
childhood
Childhood leukemia
Children
cytogenetics
Cytogenetics - methods
Flow Cytometry - methods
Flow-sorting
Fluorescence in situ hybridization
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Humans
Immunoglobulin G - immunology
In Situ Hybridization, Fluorescence - methods
lymphocytic leukemia
Minimal residual disease
Molecular immunology
Neoplasm, Residual - chemistry
Neoplasm, Residual - immunology
PCR
polymerase chain reaction
Polymerase Chain Reaction - methods
Precursor Cell Lymphoblastic Leukemia-Lymphoma - genetics
Precursor Cell Lymphoblastic Leukemia-Lymphoma - immunology
Precursor Cell Lymphoblastic Leukemia-Lymphoma - pathology
Receptors, Antigen, T-Cell - immunology
Techniques
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Description
Summary:Discordances between minimal residual disease estimates obtained by different methods are a problem in childhood acute lymphoblastic leukemia. We aimed to optimize methods allowing the biological exploration of such discrepancies, i.e. the combination of flow-sorting of small immunophenotypically defined cell populations with subsequent analyses of leukemia-associated cytogenetic and molecular marker. The approaches described here optimize the use of the same tube of unfixed, antibody-stained BM cells for flow-sorting of small cell populations and subsequent exploratory FISH and PCR-based analyses.
ISSN:0022-1759
1872-7905
DOI:10.1016/j.jim.2011.04.007