Optimization of the fermentation media for sophorolipid production from Candida bombicola ATCC 22214 using a simplex centroid design

This article describes the use of a simplex centroid mixture experimental design to optimize the fermentation medium in the production of sophorolipids (SLs) using Candida bombicola. In the first stage, 16 media ingredients were screened for the ones that have the most positive influence on the SL p...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology progress 2010-07, Vol.26 (4), p.938-944
Main Authors: Rispoli, Fred J., Badia, Daniel, Shah, Vishal
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Candida - drug effects
Candida - metabolism
Candida bombicola
Carbohydrates
Culture Media - pharmacology
Fermentation
Fermentation - drug effects
Fructose
Fundamental and applied biological sciences. Psychology
Glucose
Glycerol
Glycolipids - biosynthesis
Lactose
Lipids
media optimization
Metals
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
mineral oil
Multiple regression analysis
Nitrogen sources
Oleic acid
peptone
Phosphorus
simplex centroid design
sophorolipids
Sucrose
Sugar
Urea
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This article describes the use of a simplex centroid mixture experimental design to optimize the fermentation medium in the production of sophorolipids (SLs) using Candida bombicola. In the first stage, 16 media ingredients were screened for the ones that have the most positive influence on the SL production. The sixteen ingredients that were chosen are five different carbohydrates (fructose, glucose, glycerol, lactose, and sucrose), five different nitrogen sources (malt extract, peptone extract, soytone, urea, and yeast extract), two lipid sources (mineral oil and oleic acid), two phosphorus sources (K2HPO4 and KH2PO4), MgSO4, and CaCl2. Multiple regression analysis and centroid effect analysis were carried out to find the sugar, lipid, nitrogen source, phosphorus source, and metals having the most positive influence. Sucrose, malt extract, oleic acid, K2HPO4, and CaCl2 were selected for the second stage of experiments. An augmented simplex centroid design for five ingredients requiring 16 experiments was used for the optimization stage. This produced a quadratic model developed to help understand the interaction amongst the ingredients and find the optimal media concentrations. In addition, the top three results from the optimization experiments were used to obtain constraints that identify an optimal region. The model together with the optimal region constraints predicts the maximum production of SLs when the fermentation media is composed of sucrose, 125 g/L; malt extract, 25 g/L; oleic acid, 166.67 g/L; K2HPO4, 1.5 g/L; and CaCl2, 2.5 g/L. The optimal media was validated experimentally and a yield of 177 g/L was obtained. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2010
ISSN:8756-7938
1520-6033
1520-6033
DOI:10.1002/btpr.399