Preparation and evaluation of lactose-modified monoliths for the adsorption and decontamination of plant toxins and lectins

A series of sugar-modified porous silica monoliths with different sugar ligands (β-lactoside, β-N-acetyllactosaminide, β-d-galactoside, β-d-N-acetylgalactosaminide and β-d-glucoside) and linkers were prepared and evaluated using plant toxins and lectins including ricin and a Ricinus communis aggluti...

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Bibliographic Details
Published in:Carbohydrate research 2011-09, Vol.346 (13), p.1820-1826
Main Authors: Kato, Haruhito, Uzawa, Hirotaka, Nagatsuka, Takehiro, Kondo, Satoshi, Sato, Keita, Ohsawa, Isaac, Kanamori-Kataoka, Mieko, Takei, Yoshiyuki, Ota, Shigenori, Furuno, Masahiro, Dohi, Hirofumi, Nishida, Yoshihiro, Seto, Yasuo
Format: Article
Language:English
Subjects:
Adsorption
Decontamination
Decontamination - methods
Glycosides - chemistry
Lactose - chemistry
Lactoside
Monolith
Plant Lectins - chemistry
Plant Lectins - isolation & purification
Ricin
Ricin - chemistry
Toxins, Biological - chemistry
Toxins, Biological - isolation & purification
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Summary:A series of sugar-modified porous silica monoliths with different sugar ligands (β-lactoside, β-N-acetyllactosaminide, β-d-galactoside, β-d-N-acetylgalactosaminide and β-d-glucoside) and linkers were prepared and evaluated using plant toxins and lectins including ricin and a Ricinus communis agglutinin (RCA120). Among these sugar monoliths, a lactose monolith carrying a triethylene glycol spacer adsorbed ricin and RCA120 with the highest efficiency. The monolith showed no binding with albumin, globulin, and lectins from Jack beans, Osage orange, Amur maackia and wheat germ. All these data support the utility of the lactose-modified monolith as a tool for adsorption and decontamination of plant toxins.
ISSN:0008-6215
1873-426X
DOI:10.1016/j.carres.2011.06.025