Using a micro electroporation chip to determine the optimal physical parameters in the uptake of biomolecules in HeLa cells

In this study, a new micro electroporation (EP) cell chip with three-dimensional (3D) electrodes was fabricated by means of MEMS technology, and tested on cervical cancer (HeLa) cells. Extensive statistical data of the threshold electric field and pulse duration were determined to construct an EP “p...

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Bibliographic Details
Published in:Bioelectrochemistry (Amsterdam, Netherlands) Netherlands), 2007-05, Vol.70 (2), p.363-368
Main Authors: He, Huiqi, Chang, Donald C., Lee, Yi-Kuen
Format: Article
Language:English
Subjects:
3D electrode
Biopolymers - pharmacokinetics
Cell Membrane - metabolism
Cell Membrane - radiation effects
Cell Membrane Permeability - physiology
Cell Membrane Permeability - radiation effects
Computer Simulation
Dose-Response Relationship, Radiation
Electroporation - instrumentation
Electroporation - methods
HeLa Cells
Humans
Membrane permeability
MEMS
Micro electroporation
Models, Biological
Phase diagram
Radiation Dosage
Size effect
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Summary:In this study, a new micro electroporation (EP) cell chip with three-dimensional (3D) electrodes was fabricated by means of MEMS technology, and tested on cervical cancer (HeLa) cells. Extensive statistical data of the threshold electric field and pulse duration were determined to construct an EP “phase diagram”, which delineates the boundaries for 1) effective EP of five different size molecules and 2) electric cell lysis at the single-cell level. In addition, these boundary curves (i.e., electric field versus pulse duration) were fitted successfully with an exponential function with three constants. We found that, when the molecular size increases, the corresponding electroporation boundary becomes closer to the electric cell lysis boundary. Based on more than 2000 single-cell measurements on five different size molecules, the critical size of molecule was found to be approximately 40 kDa. Comparing to the traditional instrument, MEMS-based micro electroporation chip can greatly shorten the experimental time.
ISSN:1567-5394
1878-562X
DOI:10.1016/j.bioelechem.2006.05.008