Isolation, partial characterisation and immunomodulatory activities of polysaccharide from Morchella esculenta

BACKGROUND: Polysaccharides extracted from fungi or algae have shown a variety of medical activities, and the exploitation of polysaccharides for application in pharmacy has been very promising. In this study, the immunomodulatory properties of polysaccharides from Morchella esculenta were investiga...

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Bibliographic Details
Published in:Journal of the science of food and agriculture 2011-09, Vol.91 (12), p.2180-2185
Main Authors: Cui, Hua-Li, Chen, Yan, Wang, Shu-Sheng, Kai, Gui-Qing, Fang, Yu-Ming
Format: Article
Language:English
Subjects:
Adjuvants, Immunologic - administration & dosage
Adjuvants, Immunologic - chemistry
Adjuvants, Immunologic - isolation & purification
Adjuvants, Immunologic - pharmacology
Algae
Animals
Animals, Outbred Strains
Ascomycota - metabolism
Biological and medical sciences
Carbohydrates
Cell Proliferation - drug effects
Cells, Cultured
Culture Media, Conditioned - chemistry
Detectors
Dose-Response Relationship, Drug
Drug Discovery
Evaporative
Fermentation
Food industries
Functional foods & nutraceuticals
Fundamental and applied biological sciences. Psychology
Fungi
Immune system
Immunomodulatory activity
Light scattering
Liquid chromatography
Lymphocyte Activation - drug effects
Lymphocytes - drug effects
Lymphocytes - immunology
Macrophage Activation - drug effects
Macrophages, Peritoneal - drug effects
Macrophages, Peritoneal - immunology
Macrophages, Peritoneal - metabolism
Medical
Mice
Molecular Weight
Monosaccharides - analysis
Morchella esculenta
Nitric Oxide - metabolism
Polysaccharides
Polysaccharides - administration & dosage
Polysaccharides - chemistry
Polysaccharides - isolation & purification
Polysaccharides - pharmacology
proliferation
Random Allocation
Solubility
Spleen - cytology
Spleen - drug effects
Spleen - immunology
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Summary:BACKGROUND: Polysaccharides extracted from fungi or algae have shown a variety of medical activities, and the exploitation of polysaccharides for application in pharmacy has been very promising. In this study, the immunomodulatory properties of polysaccharides from Morchella esculenta were investigated to identify immunostimulatory activity and potentially contribute to the therapeutic potential of Morchella esculenta. RESULTS: A water‐soluble polysaccharide, MEP, was obtained from the fermentation broth of Morchella esculenta. Two fractions of this polysaccharide (MEP‐I and MEP‐II) were extracted and purified. High‐performance gel permeation chromatography analysis showed the average molecular weights of two polysaccharides. Structural properties and compositions of these two fractions were examined by Fourier transform infrared and a high‐performance liquid chromatography with an evaporative light scattering detector. Active experiments suggested that the MEP had typical immunostimulatory activity. CONCLUSION: These bioactivity tests showed that the polysaccharides from Morchella esculenta presented significant immune modulating activity, and this finding may offer the basis for the popular use of polysaccharides in functional foods or medicine. Copyright © 2011 Society of Chemical Industry
ISSN:0022-5142
1097-0010
1097-0010
DOI:10.1002/jsfa.4436