Comparison of several methods for effective lipid extraction from microalgae

Various methods, including autoclaving, bead-beating, microwaves, sonication, and a 10% NaCl solution, were tested to identify the most effective cell disruption method. The total lipids from Botryococcus sp., Chlorella vulgaris, and Scenedesmus sp. were extracted using a mixture of chloroform and m...

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Bibliographic Details
Published in:Bioresource technology 2010, Vol.101 (1), p.S75-S77
Main Authors: Lee, Jae-Yon, Yoo, Chan, Jun, So-Young, Ahn, Chi-Yong, Oh, Hee-Mock
Format: Article
Language:English
Subjects:
alkali treatment
autoclaving
Biodiesel
Biomass
Bioreactors
Botryococcus
Cell disruption
Cells, Cultured
Chemical Fractionation - methods
Chlorella vulgaris
Chloroform
Chloroform - chemistry
Chlorophyta - chemistry
fatty acid composition
lipid content
Lipid extraction
Lipids
Lipids - isolation & purification
Methanol
Methanol - chemistry
Microalgae
Microwave oven
microwave treatment
Oleic acid
renewable energy sources
Scenedesmus
Sodium chloride
Solvents - chemistry
Sonication
Time Factors
ultrasonic treatment
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Summary:Various methods, including autoclaving, bead-beating, microwaves, sonication, and a 10% NaCl solution, were tested to identify the most effective cell disruption method. The total lipids from Botryococcus sp., Chlorella vulgaris, and Scenedesmus sp. were extracted using a mixture of chloroform and methanol (1:1). The lipid contents from the three species were 5.4–11.9, 7.9–8.1, 10.0–28.6, 6.1–8.8, and 6.8–10.9 g L −1 when using autoclaving, bead-beating, microwaves, sonication, and a 10% NaCl solution, respectively. Botryococcus sp. showed the highest oleic acid productivity at 5.7 mg L −1 d −1 when the cells were disrupted using the microwave oven method. Thus, among the tested methods, the microwave oven method was identified as the most simple, easy, and effective for lipid extraction from microalgae.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2009.03.058