In vitro and in vivo biocompatibility studies of a recombinant analogue of spidroin 1 scaffolds

The goal of this study was to generate porous scaffolds from the genetically engineered protein, an analogue of Nephila clavipes spidroin 1 (rS1/9) and to assess the properties of new rS1/9 scaffolds essential for bioengineering. The salt leaching technique was used to make the rS1/9 scaffolds of in...

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Published in:Journal of biomedical materials research. Part A 2011-01, Vol.96A (1), p.125-131
Main Authors: Moisenovich, M. M., Pustovalova, O. L., Yu Arhipova, A., Vasiljeva, T. V., Sokolova, O. S., Bogush, V. G., Debabov, V. G., Sevastianov, V. I., Kirpichnikov, M. P., Agapov, I. I.
Format: Article
Language:English
Subjects:
Analogue
Animals
Biocompatibility
Biocompatible Materials - chemistry
biodegradable scaffold
Bioengineering
Biomedical materials
biopolymer
Cells, Cultured
Female
Fibroblasts - cytology
Fibroins - chemistry
Fibroins - genetics
Materials Testing
Mice
Mice, Inbred BALB C
Nephila clavipes
Porosity
Recombinant
Recombinant Proteins - chemistry
Recombinant Proteins - genetics
Scaffolds
spidroin
Surgical implants
Tissue Engineering - methods
Tissue Scaffolds - chemistry
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Summary:The goal of this study was to generate porous scaffolds from the genetically engineered protein, an analogue of Nephila clavipes spidroin 1 (rS1/9) and to assess the properties of new rS1/9 scaffolds essential for bioengineering. The salt leaching technique was used to make the rS1/9 scaffolds of interconnected macroporous structure with spontaneously formed micropores. The tensile strength of scaffolds was 18 ± 5 N/cm2. Scaffolds were relatively stable in a phosphate buffer but degraded in oxidizing environment after 11 weeks of incubation. Applicability of the recombinant spidroin 1 as a substrate for cell culture was demonstrated by successful 3T3 cells growth on the surface of rS1/9 films (270 ± 20 cells/mm2 vs. 97 ± 8 cells/mm2 on the glass surface, p < 0.01). The 3T3 fibroblasts readily proliferated within the rS1/9 scaffold (from initially plated 19 ± 2 cells/mm3 to 3800 ± 304 cells/mm3 after 2 weeks). By this time, cells were uniformly distributed between the surface and deeper layers (27% ± 8% and 33% ± 4%, respectively; p > 0.05), whereas the initial distribution was 58% ± 7% and 11% ± 8%, respectively; p < 0.05). The rS1/9 scaffolds implanted subcutaneously into Balb/c mice were well tolerated. Over a 2‐month period, the scaffolds promoted an ingrowth of de novo formed vascularized connective tissue elements and nerve fibers. Thus, scaffolds made of the novel recombinant spidroin 1 analogue are potentially applicable in tissue engineering. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.
ISSN:1549-3296
1552-4965
1552-4965
DOI:10.1002/jbm.a.32968