Evaluation of the hepatotoxic and hepatoprotective effect of Rwandese herbal drugs on in vivo (guinea pigs barbiturate-induced sleeping time) and in vitro (rat precision-cut liver slices, PCLS) models

Precision-cut liver slices (PCLS) preserve the tissular organization of the organ and represent an in vitro model closer to in vivo conditions than hepatocytes cultures. As this may be an interesting tool not only for the investigation of hepatotoxic and protective effects but also for bioguided fra...

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Bibliographic Details
Published in:Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie 2010-05, Vol.62 (3), p.289-299
Main Authors: Mukazayire, Marie-Jeanne, Allaeys, Véronique, Buc Calderon, Pedro, Stévigny, Caroline, Bigendako, Marie-Josée, Duez, Pierre
Format: Article
Language:English
Subjects:
Acetaminophen - toxicity
Analgesics, Non-Narcotic - toxicity
Animals
Barbiturates - pharmacology
Biological and medical sciences
Crassocephalum vitellinum
Disease Models, Animal
Female
Guinea Pigs
Guizotia
Guizotia scabra
Hepatoprotection
Hepatotoxicity
Hypnotics and Sedatives - pharmacology
Investigative techniques, diagnostic techniques (general aspects)
Liver - drug effects
Male
Medical sciences
Microglossa pyrifolia
Ocimum
Ocimum - chemistry
Ocimum lamiifolium
Organ Culture Techniques - methods
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Phytotherapy - methods
Plant Extracts - chemistry
Plant Extracts - pharmacology
Plant Leaves - chemistry
Plants, Medicinal - chemistry
Rat precision-cut liver slices (PCLS)
Rats
Rwanda
Sleep - drug effects
Sleeping time
Vernonia
Vernonia lasiopus
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Summary:Precision-cut liver slices (PCLS) preserve the tissular organization of the organ and represent an in vitro model closer to in vivo conditions than hepatocytes cultures. As this may be an interesting tool not only for the investigation of hepatotoxic and protective effects but also for bioguided fractionations schemes, the usefulness of PCLS was compared with an in vivo test of liver function. Crude extracts derived from five herbs used in Rwanda for hepatoprotective activity were tested on CCl4-treated guinea pigs by the method of barbiturate-induced sleep modification. Aqueous extracts of Ocimum lamiifolium, Crassocephalum vitellinum, Guizotia scabra and Vernonia lasiopus leaves allowed animals to recover barbiturate sleep duration in proportions of 88%, 78%, 61% and 34%, respectively and Microglossa pyrifolia was found inactive. Dried methanolic extracts of the 5 plants were then tested in vitro on rat PCLS for protection against acetaminophen-induced hepatotoxicity. In this model, G. scabra, M. pyrifolia and V. lasiopus were found hepatotoxic by themselves and unable to prevent acetaminophen toxicity. The most active extract, obtained from O. lamiifolium, was subjected to bioassay-guided fractionation by chromatography on Si–C18 to yield two quite active fractions. From a single animal, at least 50 PCLS explants can be prepared, which allows testing large amounts of samples, strengthening ethnopharmacological data on hepatoprotective medicinal plants and investigating hepatotoxic effects.
ISSN:0940-2993
1618-1433
DOI:10.1016/j.etp.2009.04.005