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Evaluation of lead exposure in battery-manufacturing workers with focus on different biomarkers
The influence of exposure to lead on the frequency of micronuclei (MN), nuclear buds and nucleoplasmatic bridges was investigated in peripheral blood lymphocytes in 15 male battery‐manufacturing workers and 15 controls matched for age and smoking habits. In addition to MN test, blood lead (B‐Pb) and...
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Published in: | Journal of applied toxicology 2010-05, Vol.30 (4), p.321-328 |
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Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
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Online Access: | Get full text |
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Summary: | The influence of exposure to lead on the frequency of micronuclei (MN), nuclear buds and nucleoplasmatic bridges was investigated in peripheral blood lymphocytes in 15 male battery‐manufacturing workers and 15 controls matched for age and smoking habits. In addition to MN test, blood lead (B‐Pb) and cadmium (B‐Cd), delta aminolevulinic acid dehydratase (ALAD) activity, erythrocyte protoporphyrin (EP), serum vitamin B12 (S‐Vit B12) and folate (S‐folate) were determined in all subjects. Lead‐exposed subjects had significantly higher MN frequency and B‐Pb concentrations than controls. In control smokers we found a significant negative correlation between B‐Pb concentration and frequency of nucleoplasmatic bridges, and nuclear division index. In control non‐smokers a significant positive correlation was observed only between age and nuclear buds frequency, and between S‐folate and B‐Pb level. In lead exposed smokers, significant positive correlations between MN frequency and S‐Vit B12, S‐folate, and nuclear buds frequency were found. A positive correlation in exposed smokers was also found between nuclear buds frequency and S‐Vit B12 concentration. A negative correlation was found between ALAD and EP, and B‐Pb in exposed smokers. Exposed non‐smokers showed significant negative correlation between MN frequency and B‐Cd, and ALAD and EP. The results indicate a genotoxicity of lead, pointing to a micronucleus assay as a relevant test for assessing genotoxic effects resulting from occupational exposure. The other indicators did not necessarily follow the results of THE MN test. Influence of smoking should be further investigated. Copyright © 2009 John Wiley & Sons, Ltd.
The influence of exposure to lead on the frequency of micronuclei (MN), nuclear buds and nucleoplasmatic bridges was investigated in 15 male battery‐manufacturing workers and 15 controls. The blood lead and cadmium, delta aminolevulinic acid dehydratase activity, erythrocyte protoporphyrin, serum vitamin B12 and folate were also determined. The results indicate a genotoxicity of lead, pointing to a micronucleus assay as a relevant test for assessing genotoxic effects resulting from occupational exposure. Influence of smoking should be further investigated. |
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ISSN: | 0260-437X 1099-1263 1099-1263 |
DOI: | 10.1002/jat.1497 |