Chemoprevention of a flavonoid fraction from Rhus verniciflua Stokes on aflatoxin B1-induced hepatic damage in mice

Since aflatoxin B1 (AFB1)‐mediated hepatic damage is related to the production of AFB1‐8,9‐epoxide and reactive oxygen species, bioactive compounds having antioxidant potentials are suggested to be capable of reducing AFB1‐induced toxicity. We previously purified a mixture of flavonoids that we name...

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Bibliographic Details
Published in:Journal of applied toxicology 2011-03, Vol.31 (2), p.150-156
Main Authors: Choi, Ki-Choon, Chung, Wan-Tae, Kwon, Jung-Kee, Jang, Yong-Suk, Yu, Ji-Yeon, Park, Seung-Moon, Lee, Jeong-Chae
Format: Article
Language:English
Subjects:
aflatoxin B1
Aflatoxin B1 - antagonists & inhibitors
Aflatoxin B1 - toxicity
Animals
antioxidant
Antioxidants - therapeutic use
Biological and medical sciences
Carcinogenesis, carcinogens and anticarcinogens
Cell Survival - drug effects
Chemical agents
Chemical and Drug Induced Liver Injury - immunology
Chemical and Drug Induced Liver Injury - metabolism
Chemical and Drug Induced Liver Injury - prevention & control
chemoprevention
flavonoids
Flavonoids - analysis
Flavonoids - therapeutic use
Glutathione - metabolism
Hep G2 Cells
Hepatocytes - drug effects
Hepatocytes - metabolism
Humans
Immunoglobulin Isotypes - blood
liver damage
Male
Malondialdehyde - metabolism
Medical sciences
Mice
Mice, Inbred ICR
Plant Extracts - chemistry
Plant Extracts - therapeutic use
Plant poisons toxicology
Random Allocation
Reactive Oxygen Species - metabolism
Rhus
Rhus - chemistry
Superoxide Dismutase - metabolism
Toxicology
Tumors
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Summary:Since aflatoxin B1 (AFB1)‐mediated hepatic damage is related to the production of AFB1‐8,9‐epoxide and reactive oxygen species, bioactive compounds having antioxidant potentials are suggested to be capable of reducing AFB1‐induced toxicity. We previously purified a mixture of flavonoids that we named RCMF (Rhus verniciflua Stokes chloroform–methanol fraction), from a traditional Korean food additive and herbal medicine. RCMF exhibited various biological effects, including antioxidant and antitumor activities. In this study, we examined whether RCMF protects against AFB1‐induced liver injury using in vitro and in vivo systems. Pretreatment of HepG2 cells with RCMF significantly reduced AFB1‐stimulated production of ROS and malondialdehyde (MDA) to the control levels. RCMF also prevented the reduction in HepG2 cell viability caused by AFB1. Oral administration of RCMF to mice significantly suppressed an AFB1‐induced increase in serum levels of alanine aminotransferase, alkaline phosphatase and lactate dehydrogenase. It also prevented MDA formation and blocked decreases in glutathione levels and superoxide dismutase activities in the livers of AFB1‐treated mice. In addition, RCMF supplementation prevented an AFB1‐induced decrease in serum titers of IgA and IgG1. Collectively, these results suggest that RCMF attenuates AFB1‐mediated damage to the liver, and that this effect is at least partially related to the restoration of antioxidant defense systems and an increase in AFB1–GSH conjugate formation. Copyright © 2010 John Wiley & Sons, Ltd. Since aflatoxin B1 (AFB1)‐mediated hepatic damage is related to the production of AFB1‐8,9‐epoxide and reactive oxidants, bioactive compounds having antioxidant potentials are suggested to be capable of reducing AFB1‐induced toxicity. This study examined whether a mixture of flavonoids, named RCMF, protects against AFB1‐induced liver injury. Herein we demonstrate that RCMF attenuates AFB1‐mediated damage to the liver, and that this effect is at least partially related to the restoration of antioxidant defense systems and an increase in AFB1‐GSH conjugate formation.
ISSN:0260-437X
1099-1263
1099-1263
DOI:10.1002/jat.1575