The effect of the association of near infrared laser therapy, bone morphogenetic proteins, and guided bone regeneration on tibial fractures treated with internal rigid fixation: A Raman spectroscopic study

Fractures have different etiology and treatment and may be associated or not to bone losses. Laser light has been shown to improve bone healing. We aimed to assess, through Raman spectroscopy, the level of CHA (∼958 cm−1) on complete fractures animals treated with IRF treated or not with Low Level L...

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Published in:Journal of biomedical materials research. Part A 2010-09, Vol.94A (4), p.1257-1263
Main Authors: Lopes, Cibele B., Pacheco, Marcos T. T., Silveira Jr, Landulfo, Cangussú, Maria Cristina T., Pinheiro, Antonio L. B.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
biomaterials
bone healing
Bone Morphogenetic Proteins - pharmacology
Bone Morphogenetic Proteins - therapeutic use
Bone Regeneration - drug effects
Bone Regeneration - physiology
Cattle
Diseases of the osteoarticular system. Orthopedic treatment
Fracture Fixation, Internal - methods
Guided Tissue Regeneration - methods
Infrared Rays
Laser Therapy
Male
Medical sciences
miniplates
Orthopedic surgery
Rabbits
Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)
Spectrum Analysis, Raman
Surface Properties - drug effects
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Tibial Fractures - drug therapy
Tibial Fractures - surgery
traumatology
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Summary:Fractures have different etiology and treatment and may be associated or not to bone losses. Laser light has been shown to improve bone healing. We aimed to assess, through Raman spectroscopy, the level of CHA (∼958 cm−1) on complete fractures animals treated with IRF treated or not with Low Level Laser Therapy—LLLT and associated or not to BMPs and GBR. Complete tibial fractures were created on 15 animals that were divided into five groups. LLLT (Laser Unit, Kondortech, São Carlos, SP, Brazil, λ790 nm, 4 J/cm2/point, 40 mW, ϕ ∼ 0.5 cm2, 16 J/cm2 session) started immediately after surgery and repeated at 48 h interval (2 weeks). Animal death occurred after 30 days. Raman spectroscopy was performed at the surface of the fracture. Our results showed significant differences between the groups IRF + BL /IRF_NBL (p = 0.05); between all experimental groups and untreated bone; bone/IRF + BL; IRF + BL + Bio + GBR; IRF + BL + LLLT; IRF + BL + Bio + GBR + LLLT; IRF_NBL (p < 0.001, all); IRF_NBL/IRF + BL + LLLT (p = 0.03); IRF_NBL/IRF + BL + Bio + GBR + LLLT (p = 0.02); IRF + BL/IRF + BL + LLLT (p = 0.04); IRF + BL/IRF + BL + Bio + GBR + LLLT (p = 0.002); IRF + BL + Bio + GBR/IRF + BL + Bio + GBR + LLLT (p = 0.05). It is concluded that the use of NIR LLLT associated to BMPs and GBR was effective in improving bone healing on fractured bones due to increased levels of CHA. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.
ISSN:1549-3296
1552-4965
1552-4965
DOI:10.1002/jbm.a.32800