Constitutive expression and enzymatic activity of Tan protein in brain and epidermis of Ceratitis capitata and of Drosophila melanogaster wild-type and tan mutants

The present report shows a partial biochemical characterization and life cycle expression of N-β-alanyldopamine hydrolase (Tan protein) in Ceratitis capitata and Drosophila melanogaster. This enzyme catalyzes the hydrolysis of N-β-alanyldopamine (NBAD), the main tanning precursor of insect brown cut...

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Bibliographic Details
Published in:Insect biochemistry and molecular biology 2011-09, Vol.41 (9), p.653-659
Main Authors: Pérez, M.M., Sabio, G., Badaracco, A., Quesada-Allué, L.A.
Format: Article
Language:English
Subjects:
adults
Animals
Base Sequence
Brain
Brain - enzymology
Ceratitis capitata
Ceratitis capitata - enzymology
Ceratitis capitata - growth & development
Chromosomal Proteins, Non-Histone - genetics
Chromosomal Proteins, Non-Histone - metabolism
Cuticles
Dermis - enzymology
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Dopamine
Drosophila melanogaster
Drosophila melanogaster - enzymology
Drosophila melanogaster - growth & development
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Embryos
Enzymatic activity
enzyme activity
Epidermis
gene expression
Gene Expression Regulation, Developmental
Histamine
hydrolase
Hydrolysis
Insect Proteins - genetics
Insect Proteins - metabolism
insects
Larva - enzymology
Life cycle
Metabolism
Molecular Sequence Data
mutants
N-β-alanyldopamine
NBAD-hydrolase
NBAD-synthase
Neurotransmitters
Optic lobe
Phenotype
Pupa - enzymology
Recycling
sclerotization
Tanning
β-alanylderivatives
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Summary:The present report shows a partial biochemical characterization and life cycle expression of N-β-alanyldopamine hydrolase (Tan protein) in Ceratitis capitata and Drosophila melanogaster. This enzyme catalyzes the hydrolysis of N-β-alanyldopamine (NBAD), the main tanning precursor of insect brown cuticles. It also plays an important role in the metabolism of brain neurotransmitters, recycling dopamine and histamine. In contrast to NBAD-synthase, Tan is expressed constitutively in epidermis and does not respond directly to microbial challenge. Immunodetection experiments showed the novel localization of NBAD-hydrolase in the embryo central neural system and in different regions of the adult brain, in addition to optic lobes. We sequenced and characterized Drosophila mutants tan 1 and tan 3 . The latter appears to be a mutant with normal expression in neural tissue but weak one in epidermis. [Display omitted] ► NBAD-hydrolase recycles neurotransmitters: dopamine, histamine and maybe others. ► Novel expression in epidermis and neural tissue is shown. ► NBAD-hydrolase activity is constitutive even in absence of NBAD-synthase activity. ► tan 1 and tan 3 mutants were characterized.
ISSN:0965-1748
1879-0240
DOI:10.1016/j.ibmb.2011.04.007