Selective separation and enrichment of peptides for MS analysis using the microspheres composed of Fe3O4SiO2 core and perpendicularly aligned mesoporous SiO2 shell

In this work, we report the development of a novel enrichment protocol for peptides by using the microspheres composed of Fe3O4SiO2 Core and perpendicularly aligned mesoporous SiO2 shell (designated Fe3O4SiO2SiO2). The Fe3O4SiO2SiO2 microspheres possess useful magnetic responsivity which makes the p...

Full description

Saved in:
Bibliographic Details
Published in:Proteomics (Weinheim) 2010-03, Vol.10 (5), p.930-939
Main Authors: Chen, Hemei, Liu, Shasha, Yang, Huili, Mao, Yu, Deng, Chunhui, Zhang, Xiangmin, Yang, Pengyuan
Format: Article
Language:English
Subjects:
Adsorption
Brain
microspheres
myoglobin
Pores
proteomics
Shells
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this work, we report the development of a novel enrichment protocol for peptides by using the microspheres composed of Fe3O4SiO2 Core and perpendicularly aligned mesoporous SiO2 shell (designated Fe3O4SiO2SiO2). The Fe3O4SiO2SiO2 microspheres possess useful magnetic responsivity which makes the process of enrichment fast and convenient. The highly ordered nanoscale pores (2 nm) and high-surface areas of the microspheres were demonstrated to have good size-exclusion effect for the adsorption of peptides. An increase of S/N ratio over 100 times could be achieved by using the microspheres to enrich a standard peptide, and the application of the microspheres to enrich universal peptides was performed by using myoglobin tryptic digest solution. The enrichment efficiency of re-used Fe3O4SiO2SiO2 microspheres was also studied. Large-scale enrichment of endogenous peptides in rat brain extract was achieved by the microspheres. Automated nano-LC-ESI-MS/MS was applied to analyze the sample after enrichment, and 60 unique peptides were identified in total. The facile and low-cost synthesis as well as the convenient and efficient enrichment process of the novel Fe3O4SiO2SiO2 microspheres makes it a promising candidate for selectively isolation and enrichment of endogenous peptides from complex biological samples.
ISSN:1615-9861
DOI:10.1002/pmic.200900553