Biological behavior of pre-osteoblasts on natural hydroxyapatite: A study of signaling molecules from attachment to differentiation

Several biomaterials have been widely used in bone regeneration in both orthopedic and oral surgeries. However, it is poorly understood how these biomaterials alter osteoblast phenotype. It prompted us to examine the involvement of signaling proteins during preosteoblast adhesion (attachment), proli...

Full description

Saved in:
Bibliographic Details
Published in:Journal of biomedical materials research. Part A 2011-05, Vol.97A (2), p.193-200
Main Authors: Zambuzzi, Willian F., Ferreira, Carmen V., Granjeiro, José M., Aoyama, Hiroshi
Format: Article
Language:English
Subjects:
3T3 Cells
Actins - chemistry
Activation
Adhesion
Alkaline Phosphatase - metabolism
Animals
Biocompatibility
Biological and medical sciences
Biomedical materials
Bones
Cattle
Cell Adhesion
Cell Differentiation
Cell Proliferation
cell signaling
Cell Survival
Cofilin 1 - chemistry
Collagen - chemistry
Differentiation
Durapatite - pharmacology
Gelatin - chemistry
Hydroxyapatite
Medical sciences
Mice
Osteoblasts - cytology
Osteoblasts - metabolism
Phosphorylation
preosteoblast
Signal Transduction
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Surgical implants
Technology. Biomaterials. Equipments
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Several biomaterials have been widely used in bone regeneration in both orthopedic and oral surgeries. However, it is poorly understood how these biomaterials alter osteoblast phenotype. It prompted us to examine the involvement of signaling proteins during preosteoblast adhesion (attachment), proliferation, and differentiation on natural hydroxyapatite (HA) from bovine bone. Our results indicated that natural HA is able to promote osteoblast adhesion, proliferation, and differentiation. The osteoblast/HA interaction requires phosphorylation of tyrosine residues of focal adhesion kinase, Src, and Paxillin upon integrin activation, which culminates in the control of cofilin phosphorylation (at serine 03) via rac‐1 activation. In part, these signaling pathways are responsible for actin‐rearrangement, responsible to adapt cell‐shape on HA particles. In regarding to osteoblast differentiation, we showed that natural HA favored extracellular matrix remodeling by stimulating matrix metalloproteinase activities and alkaline phosphatase activity. Overall, this study demonstrates that osteoblast response toward bovine bone HA is initially mediated by activation of focal adhesion components, culminating on actin‐rearrangement executed by cofilin activation via rac‐1. Moreover, bovine bone HA provided an excellent microenvironment for osteoblast activity, since adhesion to differentiation. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.
ISSN:1549-3296
1552-4965
1552-4965
DOI:10.1002/jbm.a.32933