Robust and Sensitive High-Performance Liquid Chromatographic-UV Detection Technique for the Determination of Tigecycline in Rabbit Plasma

An isocratic HPLC method with detection at 248 nm was developed and fully validated for the determination of tigecycline in rabbit plasma. Minocycline was used as an internal standard. A Hypersil BDS RP-C18 column (250 x 4.6 mm, 5 microm particle size) was used with the mobile phase phosphate buffer...

Full description

Saved in:
Bibliographic Details
Published in:Journal of AOAC International 2011-05, Vol.94 (3), p.847-856
Main Authors: ZORPAS, Kostas M, VALSAMI, Georgia N, VRYONIS, Evangelos V, SKOUTELIS, Athanasios T, ARCHONTAKI, Helen A
Format: Article
Language:English
Subjects:
Animal productions
Animals
Anti-Bacterial Agents - blood
Anti-Bacterial Agents - chemistry
Biological and medical sciences
Blood plasma
Chemical properties
Chromatography, High Pressure Liquid - methods
Composition
Food industries
Fundamental and applied biological sciences. Psychology
General aspects
High performance liquid chromatography
Methods
Methods of analysis, processing and quality control, regulation, standards
Minocycline - analogs & derivatives
Minocycline - blood
Minocycline - chemistry
Molecular Structure
Physiological aspects
Rabbits
Reproducibility of Results
Sensitivity and Specificity
Spectrophotometry, Ultraviolet - methods
Terrestrial animal productions
Tigecycline
Ultraviolet radiation
Vertebrates
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An isocratic HPLC method with detection at 248 nm was developed and fully validated for the determination of tigecycline in rabbit plasma. Minocycline was used as an internal standard. A Hypersil BDS RP-C18 column (250 x 4.6 mm, 5 microm particle size) was used with the mobile phase phosphate buffer (pH 7.10, 0.070 M)-acetonitrile (76 + 24, v/v) at a flow rate of 1.0 mL/min. The elution time of tigecycline and minocycline was approximately 8.1 and 9.9 min, respectively. Calibration curves of tigecycline were linear in the concentration range of 0.021-3.15 microg/mL in plasma. The LOD and LOQ in plasma were estimated as 7 and 21 ng/mL, respectively. The intraday and interday precision values of the method were in the range of 5.0-7.1 and 5.6-9.1%, while the corresponding accuracy values were in the ranges of 92.8-111.1 and 97.6-102.3%, respectively. At the LOQ, the intraday precision was 18.7%, while intraday and interday accuracy values were 97.3 and 98.0%, respectively. Robustness of the proposed method was studied using a Plackett-Burman experimental design. A pharmacokinetic profile is presented for confirmation of the applicability of the method to pharmacokinetic studies.
ISSN:1060-3271
1944-7922
DOI:10.1093/jaoac/94.3.847