CC Chemokine Ligand 2 and Leukemia Inhibitory Factor Cooperatively Promote Pluripotency in Mouse Induced Pluripotent Cells

The pluripotency of mouse embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) can be maintained by feeder cells, which secrete leukemia inhibitory factor (LIF). We found that feeder cells provide a relatively low concentration (25 unit/ml) of LIF, which is insufficient to maintain...

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Bibliographic Details
Published in:Stem cells (Dayton, Ohio) Ohio), 2011-08, Vol.29 (8), p.1196-1205
Main Authors: Hasegawa, Yuki, Takahashi, Naoko, Forrest, Alistair R. R., Shin, Jay W., Kinoshita, Yohei, Suzuki, Harukazu, Hayashizaki, Yoshihide
Format: Article
Language:English
Subjects:
Animals
Cell Line
Chemokine
Chemokine CCL2 - genetics
Chemokine CCL2 - metabolism
Coculture Techniques
Gene Expression
Gene Expression Profiling
Genetic Markers
Homeodomain Proteins - genetics
Homeodomain Proteins - metabolism
Induced Pluripotent Stem Cells - drug effects
Induced Pluripotent Stem Cells - physiology
Kruppel-Like Transcription Factors - genetics
Kruppel-Like Transcription Factors - metabolism
Leukemia Inhibitory Factor - pharmacology
Leukemia Inhibitory Factor - secretion
Mice
Microarray
Nanog Homeobox Protein
Oligonucleotide Array Sequence Analysis
Pluripotent stem cells
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
SOXB1 Transcription Factors - genetics
SOXB1 Transcription Factors - metabolism
STAT3 Transcription Factor - metabolism
T-Box Domain Proteins - genetics
T-Box Domain Proteins - metabolism
Transcription factors
Up-Regulation
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Summary:The pluripotency of mouse embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) can be maintained by feeder cells, which secrete leukemia inhibitory factor (LIF). We found that feeder cells provide a relatively low concentration (25 unit/ml) of LIF, which is insufficient to maintain the ESCs/iPSCs pluripotency in feeder free conditions. To identify additional factors involved in the maintenance of pluripotency, we carried out a global transcript expression profiling of mouse iPSCs cultured on feeder cells and in feeder‐free (LIF‐treated) conditions. This identified 17 significantly differentially expressed genes (adjusted p value
ISSN:1066-5099
1549-4918
DOI:10.1002/stem.673