Detection of highly pathogenic avian influenza virus infection in vaccinated chicken flocks by monitoring antibodies against non-structural protein 1 (NS1)

H5 and H7 highly pathogenic avian influenza virus (HPAIV) represent a major global concern in poultries and human health. Avian influenza (AI) vaccines are available but not preferred for field applications, primarily because vaccination interferes with sero-surveillances of AIV infection. To overco...

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Bibliographic Details
Published in:Veterinary microbiology 2011-01, Vol.147 (3), p.283-291
Main Authors: Takeyama, Natsumi, Minari, Kenji, Kajihara, Masahiro, Isoda, Norikazu, Sakamoto, Ryuichi, Sasaki, Takashi, Kokumai, Norihide, Takikawa, Noriyasu, Shiraishi, Rikiya, Mase, Masaji, Hagiwara, Junko, Kodama, Toshiaki, Imamura, Takashi, Sakaguchi, Masashi, Ohgitani, Toshiaki, Sawata, Akira, Okamatsu, Masatoshi, Muramatsu, Masatake, Tsukamoto, Kenji, Lin, Zhifeng, Tuchiya, Kotaro, Sakoda, Yoshihiro, Kida, Hiroshi
Format: Article
Language:English
Subjects:
accuracy
Animals
antibodies
Antibodies, Viral - blood
Applied microbiology
Avian influenza
Avian influenza virus
Biological and medical sciences
Chickens
disease surveillance
disease transmission
DIVA system
enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - veterinary
Fundamental and applied biological sciences. Psychology
hemagglutinins
highly pathogenic avian influenza virus
immune response
immunologic techniques
Influenza A virus
Influenza A virus - immunology
Influenza in Birds - diagnosis
Influenza in Birds - immunology
Influenza in Birds - virology
Influenza Vaccines - immunology
Microbiology
Miscellaneous
nonstructural protein 1
NS1-ELISA
pathogenicity
Poultry Diseases - diagnosis
Poultry Diseases - immunology
Poultry Diseases - virology
seroprevalence
strains
test sensitivity
vaccination
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
viral antigens
Viral Nonstructural Proteins - immunology
viral proteins
Virology
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Summary:H5 and H7 highly pathogenic avian influenza virus (HPAIV) represent a major global concern in poultries and human health. Avian influenza (AI) vaccines are available but not preferred for field applications, primarily because vaccination interferes with sero-surveillances of AIV infection. To overcome the problem, ELISA systems using non-structural protein 1 (NS1) of AIV as antigens (NS1-ELISA) have been developed to measure anti-NS1 antibodies that are raised in AIV-infected but not in vaccinated chickens. However, some AI-vaccinated chickens having a weak anti-virus immune response may subsequently be infected with AIV and spread the virus. This raises a concern for the validity of NS1-ELISA to detect AIV infection in previously vaccinated chickens. In this study, we developed NS1-ELISA and assessed its feasibility to detect HPAIV infection in chickens previously immunized with H5 or H7 AI vaccines. The results indicated that the NS1-ELISA could identify HPAIV infection in both unvaccinated and vaccinated chickens at 1 week after infection in correlation with results from time-consuming virus isolation tests. Taken together, the NS1-ELISA system would be valuable tool to define HPAIV infection when AI vaccine program is in place.
ISSN:0378-1135
1873-2542
DOI:10.1016/j.vetmic.2010.07.002