High-rate conversion of methane to methanol by Methylosinus trichosporium OB3b

► High-rate conversion of methane to methanol by Methylosinus trichosporium OB3b. ► Reactions are conducted at higher cell density in the presence of high concentrations of phosphate. ► More than 1.1g/L methanol is produced in anaerobic bottles. ► A dense silicone tube membrane aerated bioreactor is...

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Bibliographic Details
Published in:Bioresource technology 2011-08, Vol.102 (15), p.7349-7353
Main Authors: Duan, Changhong, Luo, Mingfang, Xing, Xinhui
Format: Article
Language:English
Subjects:
Aerated
Aerobiosis - drug effects
Biological and medical sciences
Bioreactors - microbiology
Bubble free aeration
Conversion
Density
Formates - pharmacology
Fundamental and applied biological sciences. Psychology
Magnesium Chloride - pharmacology
Membrane
Membranes, Artificial
Methane
Methane - metabolism
Methanol
Methanol - metabolism
Methyl alcohol
Methylosinus trichosporium
Methylosinus trichosporium - cytology
Methylosinus trichosporium - drug effects
Methylosinus trichosporium - metabolism
Methylosinus trichosporium OB3b
Phosphates
Phosphates - pharmacology
Silicones
Time Factors
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Summary:► High-rate conversion of methane to methanol by Methylosinus trichosporium OB3b. ► Reactions are conducted at higher cell density in the presence of high concentrations of phosphate. ► More than 1.1g/L methanol is produced in anaerobic bottles. ► A dense silicone tube membrane aerated bioreactor is applied to highly efficient and safe methane transformation to methanol. Methanol was produced from methane with a high conversion rate using a high cell density process with Methylosinus trichosporium OB3b in the presence of a high concentration of phosphate buffer. More than 1.1g/L methanol accumulated in the reaction media under optimized reaction conditions (17g dry cell/L, 400mmol/L phosphate, and 10mmol/L MgCl2) in the presence of 20mmol/L sodium formate. The conversion rate of methane was over 60%. About 0.95g/L methanol was produced when the biotransformation was carried out in a membrane aerated reactor into which methane and oxygen were introduced via two separate dense silicone tubing. Our results provide an efficient method and a promising process for high-rate conversion of methane to methanol.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2011.04.096