Genetic diversity of nodulating and non-nodulating rhizobia associated with wild soybean (Glycine soja Sieb. & Zucc.) in different ecoregions of China

Abstract A total of 99 bacterial isolates that originated from root nodules of Glycine soja were characterized with restriction analyses of amplified 16S ribosomal DNA and 16S–23S rDNA intergenic spacers (ITS), and sequence analyses of 16S rRNA, rpoB, atpD, recA and nodC genes. When tested for nodul...

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Bibliographic Details
Published in:FEMS microbiology ecology 2011-06, Vol.76 (3), p.439-450
Main Authors: Wu, Li Juan, Wang, Hai Qing, Wang, En Tao, Chen, Wen Xin, Tian, Chang Fu
Format: Article
Language:English
Subjects:
Animal, plant and microbial ecology
Bacteria
Bacterial Proteins - genetics
Bacteriology
Biological and medical sciences
Bradyrhizobium elkanii
Bradyrhizobium japonicum
Bradyrhizobium liaoningense
Bradyrhizobium yuanmingense
China
DNA Fingerprinting
DNA, Bacterial - genetics
DNA, Ribosomal Spacer - genetics
Ecology
Ecosystem
Fabaceae - microbiology
Fundamental and applied biological sciences. Psychology
Genes
Genetic diversity
Genetic Variation
geographic distribution
Glycine max
Glycine max - microbiology
Glycine soja
Legumes
Microbial ecology
Microbiology
Miscellaneous
N-Acetylglucosaminyltransferases - genetics
Phylogeny
Plant Root Nodulation
Plant Roots - microbiology
Polymorphism, Restriction Fragment Length
Rhizobiaceae - classification
Rhizobiaceae - genetics
Rhizobiaceae - isolation & purification
Rhizobium
Ribosomal DNA
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
Sinorhizobium fredii
Soybeans
Symbiosis
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Summary:Abstract A total of 99 bacterial isolates that originated from root nodules of Glycine soja were characterized with restriction analyses of amplified 16S ribosomal DNA and 16S–23S rDNA intergenic spacers (ITS), and sequence analyses of 16S rRNA, rpoB, atpD, recA and nodC genes. When tested for nodulation of G. soja, 72 of the isolates were effective symbionts, and these belonged to five species: Bradyrhizobium japonicum, Bradyrhizobium elkanii, Bradyrhizobium yuanmingense, Bradyrhizobium liaoningense and Sinorhizobium fredii. All of these, except some B. yuanmingense strains, also formed effective nodules on the domesticated soybean Glycine max. The remaining 27 isolates did not nodulate either host, but were identified as Rhizobium. Phylogeny nodC in the G. soja symbionts suggested that this symbiosis gene was mainly maintained by vertical gene transfer. Different nodC sublineages and rrs-ITS clusters reflected the geographic origins of isolates in this study.
ISSN:0168-6496
1574-6941
DOI:10.1111/j.1574-6941.2011.01064.x