A combined A431 cell membrane chromatography and online high performance liquid chromatography/mass spectrometry method for screening compounds from total alkaloid of Radix Caulophylli acting on the human EGFR

We have developed an online analytical method that combines A431 cell membrane chromatography (A431/CMC) with high performance liquid chromatography and mass spectrometry (LC/MS) for identifying active components from Radix Caulophylli acting on human EGFR. Retention fractions on A431/CMC model were...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2010-10, Vol.878 (28), p.2712-2718
Main Authors: Sun, Meng, Ren, Jing, Du, Hui, Zhang, Yanmin, Zhang, Jie, Wang, Sicen, He, Langchong
Format: Article
Language:English
Subjects:
Active control
Alkaloids - chemistry
Alkaloids - metabolism
Alkaloids - pharmacology
Analysis
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Caulophyllum - chemistry
Cell Line, Tumor
Cell Membrane - chemistry
Cell Membrane - metabolism
Cell membrane chromatography (CMC)
Chromatography
Chromatography, High Pressure Liquid - instrumentation
Chromatography, High Pressure Liquid - methods
Drug Evaluation, Preclinical - methods
Drugs, Chinese Herbal - chemistry
Drugs, Chinese Herbal - metabolism
Drugs, Chinese Herbal - pharmacology
EGFR
Fluorenes - chemistry
Fluorenes - metabolism
Fluorenes - pharmacology
Fundamental and applied biological sciences. Psychology
General pharmacology
High performance liquid chromatography
High performance liquid chromatography/mass spectrometry (LC/MS)
Human
Humans
Hydrogen Bonding
Mass spectrometry
Mass Spectrometry - methods
Mathematical models
Medical sciences
On-line systems
Online
Pharmacology. Drug treatments
Plant Roots - chemistry
Protein Binding
Radix Caulophylli
Receptor, Epidermal Growth Factor - agonists
Receptor, Epidermal Growth Factor - antagonists & inhibitors
Receptor, Epidermal Growth Factor - metabolism
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Summary:We have developed an online analytical method that combines A431 cell membrane chromatography (A431/CMC) with high performance liquid chromatography and mass spectrometry (LC/MS) for identifying active components from Radix Caulophylli acting on human EGFR. Retention fractions on A431/CMC model were captured onto an enrichment column and the components were directly analyzed by combining a 10-port column switcher with an LC/MS system for separation and preliminary identification. Using Sorafenib tosylate as a positive control, taspine and caulophine from Radix Caulophylli were identified as the active molecules which could act on the EGFR. This A431/CMC-online-LC/MS method can be applied for screening active components acting on EGFR from traditional Chinese medicines exemplified by Radix Caulophylli and will be of great utility in drug discovery using natural medicinal herbs as a source of novel compounds.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2010.08.010