Characteristics of the antitumor activities in tumor cells and modulation of the inflammatory response in RAW264.7 cells of a novel antimicrobial peptide, chrysophsin-1, from the red sea bream ( Chrysophrys major)

► Chrysophsin-1 induced cell membrane rupture much like a lytic peptide. ► Chrysophsin-1 induced necrosis with high-concentration treatment. ► Chrysophsin-1 inhibited tumor cell growth. ► The modulatory activity of the inflammatory response and antitumor activity of chrysophsin-1 were examined. The...

Full description

Saved in:
Bibliographic Details
Published in:Peptides (New York, N.Y. : 1980) N.Y. : 1980), 2011-05, Vol.32 (5), p.900-910
Main Authors: Hsu, Jung-Chieh, Lin, Li-Ching, Tzen, Jason T.C., Chen, Jyh-Yih
Format: Article
Language:English
Subjects:
Animals
anti-infective properties
Antimicrobial Cationic Peptides - pharmacology
antimicrobial peptides
antineoplastic agents
Antineoplastic Agents - pharmacology
apoptosis
Apoptosis - drug effects
Bacteria
Biological and medical sciences
Blotting, Western
Bream
carcinoma
Caspase 8 - metabolism
Caspase 9 - metabolism
caspase-3
Cell Line
Cell Line, Tumor
Cell Membrane - drug effects
Cell Membrane - ultrastructure
cell membranes
cells
Chrysophrys major
Chrysophsin-1
cytotoxicity
fibrosarcoma
Fundamental and applied biological sciences. Psychology
Gram-positive bacteria
HeLa Cells
Humans
Immunohistochemistry
inflammation
Inflammatory response
L-Lactate Dehydrogenase - metabolism
lipopolysaccharides
lymphoma
Lytic peptide
Membranes
Mice
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Modulation
neoplasm cells
Neoplasms - metabolism
Pagrus major
Peptides
Polymerase Chain Reaction
scanning electron microscopy
Sea Bream - metabolism
secretion
Secretions
TNF-α
transmission electron microscopy
Tumor cell inhibition
tumor necrosis factor-alpha
Tumor Necrosis Factor-alpha - metabolism
Tumors
Vertebrates: endocrinology
Western blotting
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:► Chrysophsin-1 induced cell membrane rupture much like a lytic peptide. ► Chrysophsin-1 induced necrosis with high-concentration treatment. ► Chrysophsin-1 inhibited tumor cell growth. ► The modulatory activity of the inflammatory response and antitumor activity of chrysophsin-1 were examined. The antimicrobial peptide, chrysophsin-1, exhibits antimicrobial activities with similar efficiencies for both gram-negative and gram-positive bacteria. In this study, we examined the antitumor activity and modulation of the inflammatory response of a synthetic chrysophsin-1 peptide. In vitro results showed that chrysophsin-1 had greater inhibitory effects against human fibrosarcoma (HT-1080), histiocytic lymphoma (U937), and epithelial carcinoma (HeLa) cells. LDH release by HeLa cells was comparable to that of an MTS assay after treatment with 1.5–3 μg/ml chrysophsin-1 for 24 h. Under SEM and TEM observations, we found no intact cell membranes after chrysophsin-1 treatment of HeLa cells for 8 h. The suggested mechanism of the cytotoxic activity of chrysophsin-1 was disruption of cancer cell membranes. In addition, we also examined caspase-3, -8, and -9 activities by Western blotting; the results excluded the participation of apoptosis in chrysophsin-1's effect on HeLa cells. Stimulation by lipopolysaccharide induced tumor necrosis factor (TNF)-α which was able to modulate chrysophsin-1 treatment of RAW264.7 cells and inhibited endogenous TNF-α release but did not block its secretion. With data from this study, we demonstrate that chrysophsin-1 has antimicrobial and antitumor activities and modulates the inflammatory response in RAW264.7 cells.
ISSN:0196-9781
1873-5169
DOI:10.1016/j.peptides.2011.02.013