Feruloylated and Nonferuloylated Arabino-oligosaccharides from Sugar Beet Pectin Selectively Stimulate the Growth of Bifidobacterium spp. in Human Fecal in Vitro Fermentations

The side chains of the rhamnogalacturonan I fraction in sugar beet pectin are particularly rich in arabinan moieties, which may be substituted with feruloyl groups. In this work the arabinan-rich fraction resulting from sugar beet pulp based pectin production was separated by Amberlite XAD hydrophob...

Full description

Saved in:
Bibliographic Details
Published in:Journal of agricultural and food chemistry 2011-06, Vol.59 (12), p.6511-6519
Main Authors: Holck, Jesper, Lorentzen, Andrea, Vigsnæs, Louise K, Licht, Tine R, Mikkelsen, Jørn D, Meyer, Anne S
Format: Article
Language:English
Subjects:
Arabinose - chemistry
Arabinose - metabolism
Beta vulgaris - chemistry
Bifidobacterium
Bifidobacterium - growth & development
Bifidobacterium - metabolism
Bioactive Constituents
bioactive properties
Biological and medical sciences
Clostridium difficile
feces
Feces - microbiology
Fermentation
Food industries
Fruit and vegetable industries
Fundamental and applied biological sciences. Psychology
Humans
hydrophobic bonding
Intestines - metabolism
Intestines - microbiology
Models, Biological
Molecular Structure
Oligosaccharides - chemistry
Oligosaccharides - isolation & purification
Oligosaccharides - metabolism
pathogens
pectins
Pectins - chemistry
Pectins - metabolism
Plant Extracts - chemistry
Plant Extracts - isolation & purification
Plant Extracts - metabolism
Prebiotics - analysis
sugar beet
sugar beet pulp
Sugar industries
volunteers
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The side chains of the rhamnogalacturonan I fraction in sugar beet pectin are particularly rich in arabinan moieties, which may be substituted with feruloyl groups. In this work the arabinan-rich fraction resulting from sugar beet pulp based pectin production was separated by Amberlite XAD hydrophobic interaction and membrane separation into four fractions based on feruloyl substitution and arabino-oligosaccharide chain length: short-chain (DP 2–10) and long-chain (DP 7–14) feruloylated and nonferuloylated arabino-oligosaccharides, respectively. HPAEC, SEC, and MALDI-TOF/TOF analyses of the fractions confirmed the presence of singly and doubly substituted feruloylated arabino-oligosaccharides in the feruloyl-substituted fractions. In vitro microbial fermentation by human fecal samples (n = 6 healthy human volunteers) showed a selective stimulation of bifidobacteria by both the feruloylated and the nonferuloylated long-chain arabino-oligosaccharides to the same extent as the prebiotic fructo-oligosaccharides control. None of the fractions stimulated the growth of the potential pathogen Clostridium difficile in monocultures. This work provides a first report on the separation of potentially bioactive feruloylated arabino-oligosaccharides from sugar beet pulp and an initial indication of the potentially larger bifidogenic effect of relatively long-chain arabino-oligosaccharides as opposed to short-chain arabino-oligosaccharides.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf200996h