Clinicopathological and prognostic significance of Bmi-1 expression in human cervical cancer

Objective. To investigate the clinical significance of Bmi‐1 expression as a prognostic marker for cervical cancer. Design. Retrospectively collected data from a population‐based cohort. Setting. Jiangsu Province Hospital. Population. Eighty‐eight women diagnosed with cervical carcinoma between 2000...

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Bibliographic Details
Published in:Acta obstetricia et gynecologica Scandinavica 2011-07, Vol.90 (7), p.737-745
Main Authors: MIN, LUO, DONG-XIANG, SHEN, XIAO-TONG, GUO, TING, GUAN, XIAO-DONG, CHEN
Format: Article
Language:English
Subjects:
Adenocarcinoma - genetics
Adenocarcinoma - mortality
Adenocarcinoma - pathology
Aged
Analysis of Variance
Bim-1
Biological and medical sciences
Biomarkers, Tumor - analysis
Biopsy, Needle
Biotechnology
Blotting, Western
Carcinoma, Squamous Cell - genetics
Carcinoma, Squamous Cell - mortality
Carcinoma, Squamous Cell - pathology
Cervical cancer
China
Cohort Studies
Confidence Intervals
Female
Female genital diseases
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Neoplastic
Genetic engineering
Genetic technics
Gynecology. Andrology. Obstetrics
Humans
Immunohistochemistry
Kaplan-Meier Estimate
Medical sciences
Methods. Procedures. Technologies
Middle Aged
Modification of gene expression level
Multivariate Analysis
Neoplasm Staging
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Polycomb Repressive Complex 1
Prognosis
Proportional Hazards Models
Proto-Oncogene Proteins - genetics
Proto-Oncogene Proteins - metabolism
Repressor Proteins - genetics
Repressor Proteins - metabolism
Retrospective Studies
Reverse Transcriptase Polymerase Chain Reaction
Risk Assessment
RNA interference
RNA, Messenger - analysis
Sensitivity and Specificity
Statistics, Nonparametric
Survival Analysis
Tumors
Uterine Cervical Neoplasms - genetics
Uterine Cervical Neoplasms - mortality
Uterine Cervical Neoplasms - pathology
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Summary:Objective. To investigate the clinical significance of Bmi‐1 expression as a prognostic marker for cervical cancer. Design. Retrospectively collected data from a population‐based cohort. Setting. Jiangsu Province Hospital. Population. Eighty‐eight women diagnosed with cervical carcinoma between 2000 and 2003. Methods. RT‐PCR assay was performed to determine Bmi‐1 mRNA expression in 18 cervical cancer and noncancerous tissue samples and immunohistochemistry to detect Bmi‐1 protein expression in 88 cervical cancer samples. The correlation between Bmi‐1 expression and clinicopathological factors was analyzed. Additionally, statistical analyses were applied to test for prognostic associations. RNA interference was used to downregulate Bmi‐1 expression in a cervical cancer cell line (HeLa). In vitro cytotoxicity was measured by the methylthiazoletetrazolium and colony formation assays. Effects of Bmi‐1 inhibition on in vivo growth of cancer cells was detected by the tumorigenicity assay. Cell cycle distribution and cell apoptosis were measured by flow cytometry. Main Outcome Measures. The levels of Bmi‐1 mRNA and protein expression in tissues were evaluated by RT‐PCR and Western Blot assays. Results. The level of Bmi‐1 mRNA expression in cervical cancer tissues was significantly higher than that in corresponding noncancerous tissues. High Bmi‐1 expression was significantly correlated with poor tumor differentiation, advanced International Federation of Gynecology and Obstetrics stage and positive lymph node metastasis. Patients with high Bmi‐1 expression showed shorter overall survival than those with low expression. Univariate and multivariate analyses showed that high Bmi‐1 expression was an independent prognostic factor. Conclusions. RNA interference‐mediated Bmi‐1 inhibition could inhibit in vitro and in vivo growth, enhance apoptosis and induce cell cycle arrest of cervical cancer cells. Bmi‐1 might be an independent prognostic marker for cervical cancer patients.
ISSN:0001-6349
1600-0412
DOI:10.1111/j.1600-0412.2011.01102.x