The establishment of a sensitive method in determining different neurotransmitters simultaneously in rat brains by using liquid chromatography–electrospray tandem mass spectrometry

An effective way to determine the amount of different neurotransmitters is vital to the study of brain function. Here, a highly sensitive HPLC–MS/MS method was developed to simultaneously measure γ-aminobutyric acid, dopamine, epinephrine, norepinepherine, glutamate and serotonin in one sample. The...

Full description

Saved in:
Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2011-04, Vol.879 (11), p.737-742
Main Authors: Zhu, Kevin Y., Fu, Qiang, Leung, K. Wing, Wong, Zack C.F., Choi, Roy C.Y., Tsim, Karl W.K.
Format: Article
Language:English
Subjects:
Analysis
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Brain Chemistry
Chromatography, Liquid - methods
Dopamine - analysis
Dopamine - isolation & purification
Drug Stability
Embryo, Mammalian
Epinephrine - analysis
Epinephrine - isolation & purification
Fundamental and applied biological sciences. Psychology
gamma-Aminobutyric Acid - analysis
gamma-Aminobutyric Acid - isolation & purification
General pharmacology
Glutamic Acid - analysis
Glutamic Acid - isolation & purification
LC–MS
Linear Models
Male
Medical sciences
Neurotransmitter Agents - analysis
Neurotransmitter Agents - isolation & purification
Neurotransmitters analysis
Norepinephrine - analysis
Norepinephrine - isolation & purification
Pharmacology. Drug treatments
Rat brain
Rats
Rats, Sprague-Dawley
Reproducibility of Results
Sensitivity and Specificity
Serotonin - analysis
Serotonin - isolation & purification
Spectrometry, Mass, Electrospray Ionization
Tandem Mass Spectrometry - methods
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An effective way to determine the amount of different neurotransmitters is vital to the study of brain function. Here, a highly sensitive HPLC–MS/MS method was developed to simultaneously measure γ-aminobutyric acid, dopamine, epinephrine, norepinepherine, glutamate and serotonin in one sample. The quantification of the neurotransmitters was achieved by a tandem mass spectrometer using the selected reaction monitoring scan mode. The method validation included selectivity, linearity, accuracy, precision, stability, recovery and matrix effect. For the six neurotransmitters, the linear regression analysis was calibrated by deuterated internal standards with a R 2 of over 0.991, and the limit of detection (LOD) and the limit of quantification (LOQ) were from 2.5 to 500 pg/mg and 7.5 to 1000 pg/mg, respectively. This method was employed here to reveal different types and amounts of neurotransmitters simultaneously in adult and embryonic rat brains. Here, the change of dopamine concentration in embryonic and adult brain was from 0.071 to 0.760 ng/mg of brain tissue, GABA was from 207.643 to 445.148 ng/mg, glutamate was from 679.535 to 1408.920 ng/mg, serotonin was from 0.058 to 0.485 ng/mg and norepinepherine was from 0.054 to 0.290 ng/mg. For epinephrine, it was only detected in embryonic stage but not in adult, with the concentration at 0.241 ng/mg.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2011.02.011