Fast DNA hybridization on a microfluidic mixing device based on pneumatic driving

A novel fluid mixing strategy was developed which significantly enhanced the efficiency of DNA hybridization. A pneumatic micro-mixing device consisting of two pneumatic chambers and an underneath DNA microarray chamber was built up. The fluid in the array chamber was pneumatically pumped alternatel...

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Bibliographic Details
Published in:Talanta (Oxford) 2011-04, Vol.84 (2), p.565-571
Main Authors: Wang, Xin, Chen, Xuemin, Ma, Xiufeng, Kong, Xiangwei, Xu, Zhangrun, Wang, Jianhua
Format: Article
Language:English
Subjects:
Analytical chemistry
Chaotic flow
Chemistry
DNA - chemistry
DNA hybridization
Exact sciences and technology
General, instrumentation
Microfluidics
Microfluidics - instrumentation
Nucleic Acid Hybridization
Pneumatic pumping
Rapid mixing
Spectrometric and optical methods
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Summary:A novel fluid mixing strategy was developed which significantly enhanced the efficiency of DNA hybridization. A pneumatic micro-mixing device consisting of two pneumatic chambers and an underneath DNA microarray chamber was built up. The fluid in the array chamber was pneumatically pumped alternately by the two pneumatic chambers. The chaotic oscillatory flow caused by the pumping greatly intensified the fluidic mixing. A homogeneous distribution of the tracer dye solution in the microarray chamber was observed after 2s mixing with a pumping frequency of 24Hz. Microarray DNA hybridization was substantially accelerated using this device, and the fluorescence intensity showed a plateau after oscillating 30s at room temperature. The corresponding signal level of the dynamic hybridization was 12.5-fold higher than that of the static hybridization performed at 42°C. A signal-to-noise ratio of 117 was achieved and the nonspecific adsorption of the targets to the sample array was minimized, which might be attributed to the strong shearing force generated during the pneumatic mixing process.
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2011.01.065