Physiological role of carbon dioxide in spore germination of Clostridium perfringens S40

Germination of Clostridium perfringens is known to be triggered by nutrients such as l-alanine and inosine, and facilitated by CO 2, however the role of CO 2 has not been fully understood. During the studies of the germination-specific protease GSP, we found that CO 2 could be replaced by bicarbonat...

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Bibliographic Details
Published in:Journal of bioscience and bioengineering 2009-12, Vol.108 (6), p.477-483
Main Authors: Kato, Shiro, Masayama, Atsushi, Yoshimura, Tohru, Hemmi, Hisashi, Tsunoda, Hidenori, Kihara, Tomoko, Moriyama, Ryuichi
Format: Article
Language:English
Subjects:
Alanine - genetics
Amino Acid Sequence
Anaerobic bacteria
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Bacterial spore
Bicarbonate
Biological and medical sciences
Biotechnology
CARBON DIOXIDE
Carbon Dioxide - physiology
CLOSTRIDIUM PERFRINGENS
Clostridium perfringens - enzymology
Clostridium perfringens - genetics
Clostridium perfringens - physiology
DIOXIDO DE CARBONO
DIOXYDE DE CARBONE
Electrophoresis, Polyacrylamide Gel
Endopeptidases - genetics
Escherichia coli - genetics
Escherichia coli - metabolism
ESPORAS
FONCTION PHYSIOLOGIQUE
FUNCION FISIOLOGICA
Fundamental and applied biological sciences. Psychology
Gene Deletion
GERMINACION
GERMINATION
Heat-Shock Proteins - genetics
http://www.fao.org/aos/agrovoc#c_1302
http://www.fao.org/aos/agrovoc#c_32272
http://www.fao.org/aos/agrovoc#c_3247
http://www.fao.org/aos/agrovoc#c_5837
http://www.fao.org/aos/agrovoc#c_7324
Hydrolases - genetics
Hydrolases - metabolism
Microbial Viability - genetics
Molecular Sequence Data
PHYSIOLOGICAL FUNCTIONS
Protein Conformation
Recombinant Fusion Proteins - metabolism
Sequence Homology, Amino Acid
Serine Proteases - biosynthesis
Serine Proteases - genetics
SPORE
SPORES
Spores, Bacterial - enzymology
Spores, Bacterial - genetics
Spores, Bacterial - physiology
Substrate Specificity
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Summary:Germination of Clostridium perfringens is known to be triggered by nutrients such as l-alanine and inosine, and facilitated by CO 2, however the role of CO 2 has not been fully understood. During the studies of the germination-specific protease GSP, we found that CO 2 could be replaced by bicarbonate or weakly acidic pH (pH 6.0–6.5). We also found that the spores obtained from the C. perfringens S40 overproducing GSP could germinate without CO 2. Moreover, the spores could germinate in the absence of nutrients, when the spores were incubated with bicarbonate or under weakly acidic pH. GSP, which might consist of three homologous proteases, CspA, CspB, and CspC, is one of the key enzymes involved in the spore germination, and converts the pre-mature form of the spore cortex-lytic enzyme, SleC, to the mature form. Maturation of SleC in the spores obtained from the mother strain of C. perfringens S40 requires nutrients plus bicarbonate or weakly acidic pH. In contrast, mature SleC was found in the spores obtained from the cells overpoducing GSP, when the spores were treated by nutrients, bicarbonate or weakly acidic pH. Each nutrients, bicarbonate and weakly acidic pH can trigger the germination of the spores obtained from C. perfringens cells overproducing GSP.
ISSN:1389-1723
1347-4421
DOI:10.1016/j.jbiosc.2009.06.011