Characterization of the developed antimicrobial urological catheters

Antimicrobial urological catheters were developed by the mixed, covalent and non-covalent binding of sparfloxacin (SPA) to heparin (HP) film which was first deposited on the latex surface of biomaterial. The SPA-HP modified surface was characterized by SEM analysis and ATR-Fourier transform infrared...

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Bibliographic Details
Published in:International journal of pharmaceutics 2010-12, Vol.402 (1), p.175-183
Main Authors: Kowalczuk, Dorota, Ginalska, Grażyna, Golus, Joanna
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - chemistry
Anti-Bacterial Agents - pharmacology
Antibacterial activity
Antibiotics
Anticoagulants - chemistry
Anticoagulants - pharmacology
Antimicrobial activity
Antimicrobial agents
Antimicrobial protection
Biofilms
Biofilms - growth & development
Biological and medical sciences
Biomaterials
Catheters
Catheters, Indwelling - microbiology
Colonization
Diffusion
Escherichia coli
Escherichia coli - drug effects
FliM protein
Fluoroquinolones - chemistry
Fluoroquinolones - pharmacology
General pharmacology
Heparin
Heparin - chemistry
Heparin - pharmacology
I.R. spectroscopy
Immobilization
Latex
Medical sciences
Microscopy, Electron, Scanning
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
Sparfloxacin
Spectroscopy, Fourier Transform Infrared
Stains
Staphylococcus aureus
Staphylococcus aureus - drug effects
Staphylococcus epidermidis
Staphylococcus epidermidis - drug effects
Surface characterization
Time Factors
Urinary Catheterization - methods
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Description
Summary:Antimicrobial urological catheters were developed by the mixed, covalent and non-covalent binding of sparfloxacin (SPA) to heparin (HP) film which was first deposited on the latex surface of biomaterial. The SPA-HP modified surface was characterized by SEM analysis and ATR-Fourier transform infrared spectroscopy. For the antimicrobial prevention, SPA as an antibiotic with a broad antimicrobial spectrum was chosen. Antimicrobial activity of antibiotic-modified catheter against Staphylococcus aureus, Staphylococcus epidermidis and Escherichia coli strains was assessed using various procedures. On the basis of the inhibition zone and diffusion assays the efficacy around the modified catheters was demonstrated. The test samples clearly showed an antibacterial activity against all tested bacterial stains for a least one month. Inhibition of the bacterial colonization on the modified catheter surface was proved by the biofilm test. Antimicrobial activity of SPA-treated catheter surface was also quantitatively evaluated according to standard method of ISO based on JIS. The R-values were found to be higher than 3.8. The performed research indicated that the immobilization of SPA on the catheter surface by means of the mixed-type bonds resulted in stable antibacterial protection of the urological catheters for a long time.
ISSN:0378-5173
1873-3476
DOI:10.1016/j.ijpharm.2010.10.014