Simultaneous quantification of polyherbal formulations containing Rhodiola rosea L. and Eleutherococcus senticosus Maxim. using rapid resolution liquid chromatography (RRLC)

An RRLC method capable of simultaneous identification and rapid quantification of six biologically active compounds (salidroside, tyrosol, rosarin, rosavin, rosin, rosiridin) in Rhodiola rosea L. and two active compounds (eleutheroside B and eleutheroside E) in Eleutherococcus senticosus Maxim. was...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2011-07, Vol.55 (5), p.908-915
Main Authors: Ma, Yuan-Chun, Wang, Xiao-Qiang, Hou, FeiFei, Ma, Jie, Luo, Mai, Lu, Shane, Jin, Peter, Chen, Alice, Xu, Iris, Patel, Asmita V., Gorecki, Derek
Format: Article
Language:English
Subjects:
acetonitrile
Analysis
Analytical, structural and metabolic biochemistry
bioactive properties
Biological and medical sciences
Calibration
Chemistry Techniques, Analytical
Chemistry, Pharmaceutical - methods
Chromatography - methods
Chromatography, Liquid - methods
Disaccharides - analysis
Eleutherococcus - metabolism
Eleutherococcus senticosus
Fundamental and applied biological sciences. Psychology
General pharmacology
Glucosides - analysis
herbs
ingredients
Lignans - analysis
liquid chromatography
Medical sciences
Pharmacology. Drug treatments
Phenols - analysis
Phenylethyl Alcohol - analogs & derivatives
Phenylethyl Alcohol - analysis
Phenylpropionates - analysis
Plant Extracts - analysis
Plant Extracts - pharmacology
Plant Preparations - analysis
Polyherbal formulation
Quality Control
Rapid resolution liquid chromatography (RRLC)
Reproducibility of Results
Resins, Plant - analysis
Rhodiola - metabolism
Rhodiola rosea
Rhodiola rosea L
rosin
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Summary:An RRLC method capable of simultaneous identification and rapid quantification of six biologically active compounds (salidroside, tyrosol, rosarin, rosavin, rosin, rosiridin) in Rhodiola rosea L. and two active compounds (eleutheroside B and eleutheroside E) in Eleutherococcus senticosus Maxim. was developed. The chromatographic analyses were performed on a reversed phase Phenomenex C18 (2)-HST column at 40 °C with a neutral mobile phase (purified water and acetonitrile) gradient system at a flow rate of 1.0 ml/min and UV detection at 205 and 220 nm simultaneously. Baseline separation of eight active compounds was achieved within 8 min. This developed method provides good linearity ( R > 0.9997), precision (RSD < 1.99%) and recovery of the bioactive compounds. The RRLC method developed is capable of controlling the quality of R. rosea and E. senticosus raw herbs, commercial extracts, as well as polyherbal formulations containing R. rosea and E. senticosus as ingredients. This RRLC method is accurate and sensitive; in addition, it greatly increases sample analysis throughput with reduced analysis time, which is suitable for routine quality control analysis.
ISSN:0731-7085
1873-264X
DOI:10.1016/j.jpba.2011.03.013