In vivo production of mineralised tissue pieces for clinical use: a qualitative pilot study using human dental pulp cell

Abstract Numerous previous studies have investigated the production of mineralised tissues by transplanting human dental pulp cells with calcium based scaffolds. The potential of alternative setups remains largely uninvestigated, therefore in this study, human dental pulp cells were encapsulated int...

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Bibliographic Details
Published in:International journal of oral and maxillofacial surgery 2011-06, Vol.40 (6), p.612-620
Main Authors: Chan, B, Wong, R.W.K, Rabie, B
Format: Article
Language:English
Subjects:
animal testing
Animals
Antioxidants - pharmacology
Ascorbic Acid - pharmacology
Biological and medical sciences
Calcification, Physiologic - physiology
Capillaries - pathology
Cell Culture Techniques
Cell physiology
Cell Survival
Culture Media
Dental Pulp - cytology
Dental Pulp - drug effects
Dentistry
Dexamethasone - pharmacology
Fundamental and applied biological sciences. Psychology
Glucocorticoids - pharmacology
Glycerophosphates - pharmacology
human dental pulp cell
Humans
Hydrogel, Polyethylene Glycol Dimethacrylate - chemistry
in vivo study
in vivo transplantation
Medical sciences
Mice
Mice, Nude
Mineralization, calcification
Molecular and cellular biology
Nanofibers - chemistry
Osteocalcin - analysis
osteogenesis
Osteonectin - analysis
Osteopontin - analysis
Otorhinolaryngology. Stomatology
Peptides - chemistry
Pilot Projects
Receptor, Parathyroid Hormone, Type 1 - analysis
Skeleton and joints
Stem Cells - drug effects
Stem Cells - physiology
Subcutaneous Tissue - surgery
Surgery
Time Factors
tissue engineering
Tissue Engineering - methods
Tissue Scaffolds - chemistry
Vertebrates: osteoarticular system, musculoskeletal system
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Summary:Abstract Numerous previous studies have investigated the production of mineralised tissues by transplanting human dental pulp cells with calcium based scaffolds. The potential of alternative setups remains largely uninvestigated, therefore in this study, human dental pulp cells were encapsulated into non-calcium based biomaterial – self-assembling peptide nano-fibre hydrogel. The cell-gel constructs were cultured in full medium for 2 weeks. Then they were cultured in full medium supplemented with β-glycerophosphate, dexamethasone and l -ascorbic acid for 2 more weeks. These cell-gel constructs and plain-gel constructs (with no cells) were transplanted subcutaneously into five nude mice. The gel constructs were retrieved 4 weeks after surgery. The plain-gel constructs were all completely resorbed with no new tissue formation. The cell-gel constructs were transformed into tissue pieces that were mineralised and contained blood capillaries. Immunohistochemistry analysis confirmed the expression of multiple bone markers (osteopontin, osteocalcin, osteonectin and parathyroid hormone receptor) in these tissue pieces. Computerised analysis of the contact radiographs gave the mean radio-opaque area percentage as 78% ( N = 5, P < 0.001 compared with the 0% of the control). The results demonstrate good prospects for using human dental pulp cell plus self-assembling peptide nano-fibre hydrogel to produce mineralised tissue pieces for clinical use.
ISSN:0901-5027
1399-0020
DOI:10.1016/j.ijom.2011.01.008