Functional analysis of the grapevine paralogs of the SMG7 NMD factor using a heterolog VIGS-based gene depletion-complementation system

Nonsense-mediated mRNA decay (NMD) is a eukaryotic quality control system that identifies and eliminates transcripts having a premature translation termination codon (PTC). NMD is also involved in the control of several wild-type mRNAs. The NMD core machinery consists of three highly conserved NMD f...

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Bibliographic Details
Published in:Plant molecular biology 2011-02, Vol.75 (3), p.277-290
Main Authors: Benkovics, Anna Hangyáné, Nyikó, Tünde, Mérai, Zsuzsanna, Silhavy, Dániel, Bisztray, György Dénes
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Arabidopsis - genetics
Arabidopsis - metabolism
Biochemistry
Biomedical and Life Sciences
Carrier Proteins - chemistry
Carrier Proteins - genetics
Carrier Proteins - metabolism
Conserved Sequence
Evolutionary biology
Flowers & plants
Gene Deletion
gene duplication
Gene expression
Gene Expression Regulation, Plant
Grapevine
Life Sciences
Molecular Sequence Data
Nicotiana - genetics
Nicotiana - metabolism
NMD
Phylogeny
Plant
Plant biology
Plant Pathology
Plant Sciences
Protein Binding
Proteins
Ribonucleic acid
RNA
RNA Stability
RNA, Messenger - metabolism
Sequence Alignment
SMG7
VIGS
Vitaceae
Vitis - genetics
Vitis - metabolism
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Summary:Nonsense-mediated mRNA decay (NMD) is a eukaryotic quality control system that identifies and eliminates transcripts having a premature translation termination codon (PTC). NMD is also involved in the control of several wild-type mRNAs. The NMD core machinery consists of three highly conserved NMD factors (UPF1, UPF2 and UPF3) and at least one less conserved 14-3-3-like domain containing protein (SMG7). A PTC is identified by UPF factors, and then SMG7 triggers rapid transcript decay. UPF factors are generally encoded by a single gene, whereas SMG7 has duplicated several times during evolution. Recently it was reported that the plant SMG7 is autoregulated through NMD and that SMG7 has two relatively divergent paralogs in dicots, SMG7 and SMG7L. In mammals all three SMG7 related genes (SMG5, SMG6 and SMG7) are essential in NMD, so we hypothesized that in plants the SMG7 and SMG7L duplicates may also play distinct roles in NMD. To test this possibility, we have analyzed the evolution and the function of plant SMG7 homologs. We show that SMG7L is not required for plant NMD. Interestingly, we found that the grapevine and poplar genomes contain two quite divergent SMG7 paralogs which may have derived from an ancient duplication event. Using heterolog depletion/complementation assays we demonstrate that both grapevine SMG7 copies retained the complete NMD activity and both of them are under NMD control, whilst SMG7L has lost NMD activity and NMD control.
ISSN:0167-4412
1573-5028
DOI:10.1007/s11103-010-9726-0