Development of lauroyl sulfated chitosan for enhancing hemocompatibility of chitosan

The pictorial representation of red blood cell after incubation with lauroyl sulfated chitosan (LSCS). [Display omitted] ► This work focuses on enhancing the blood compatibility of chitosan which is hemostatic in nature. ► Surface-induced thrombosis remains as one of the main problems for chitosan i...

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Bibliographic Details
Published in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2011-06, Vol.84 (2), p.561-570
Main Authors: Shelma, R., Sharma, Chandra P.
Format: Article
Language:English
Subjects:
adsorption
Agglomeration
Backbone
biopolymers
Blood
Blood Coagulation
Carbohydrate Sequence
Cell Line
Chitosan
Chitosan - chemistry
colloids
compatibility
cytotoxicity
Depletion
Derivatives
erythrocytes
Fourier transform infrared spectroscopy
Hemocompatibility
Hemolysis
Humans
hydrophilicity
hydrophobicity
Inclusions
Lauric Acids - chemistry
Lauroyl sulfated chitosan
leukocytes
Magnetic Resonance Spectroscopy
Micro particles
Molecular Sequence Data
Nanoparticles - chemistry
nuclear magnetic resonance spectroscopy
Particle Size
platelet aggregation
Protein adsorption
protein depletion
proteins
Spectroscopy, Fourier Transform Infrared
Sulfates - chemistry
thermal properties
zeta potential
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Summary:The pictorial representation of red blood cell after incubation with lauroyl sulfated chitosan (LSCS). [Display omitted] ► This work focuses on enhancing the blood compatibility of chitosan which is hemostatic in nature. ► Surface-induced thrombosis remains as one of the main problems for chitosan in blood-contacting applications. ► Hemocompatibility has been successfully achieved by the modification of chitosan with lauroyl sulfation. ► This modification will expand the applicability of chitosan in medical field. Chitosan (CS) has received much attention as a functional biopolymer especially in pharmaceutical applications, but has serious limitations owing to its poor hemo-compatibility property. Present paper focuses on the chemical modification of CS in order to enhance hemocompatibility. Amphiphilic derivative (lauroyl sulfated chitosan, LSCS) was prepared by the inclusion of sulfo group (hydrophilic) and lauroyl group (hydrophobic) to CS backbone and particles were prepared by an ionic-gellation approach. Modification was confirmed by FTIR, NMR and zeta potential measurements and the microparticles were evaluated for its particle size, swelling properties and thermal behaviour. Blood compatibility studies like hemolysis, RBC, WBC, platelet aggregation studies, blood clotting time, protein adsorption and C3 protein depletion assay were carried out for these polymers using standard techniques and cytotoxicity studies were performed to understand its applicability. Negatively charged (−6.06 mV) LSCS submicroparticles (886 nm) were prepared in this study. Blood compatibility studies demonstrated that the amphiphilic modification improved the hemocompatibility of CS. RBC aggregation and hemolysis induced by CS were significantly reduced by this modification. Further amphiphilic modification was effective in reducing the protein adsorption on CS. LSCS derivatives were found to be non-toxic in L929 cell lines. From these studies, it appears that LSCS is a hemocompatible version of CS.
ISSN:0927-7765
1873-4367
DOI:10.1016/j.colsurfb.2011.02.018