Human cytomegalovirus immediate early gene expression in the osteosarcoma line U2OS is repressed by the cell protein ATRX

The control of human cytomegalovirus (HCMV) immediate early (IE) gene expression in infected human fibroblasts was compared with that in the U2OS human osteosarcoma cells. Viral IE expression was stimulated by the virion protein pp71 and repressed by the cell protein hDaxx in fibroblasts, as expecte...

Full description

Saved in:
Bibliographic Details
Published in:Virus research 2011-04, Vol.157 (1), p.47-53
Main Authors: McFarlane, Steven, Preston, Chris M.
Format: Article
Language:English
Subjects:
ATRX
Cell Line, Tumor
chromatin
Chromatin - metabolism
Chromatin remodeling
Cytomegalovirus - genetics
Cytomegalovirus - physiology
Data processing
DNA
DNA Helicases - metabolism
Electroporation
Fibroblasts
Gene Expression
Gene Expression Regulation, Viral
Genes, Immediate-Early
genome
Genomes
Herpes simplex virus
Herpes simplex virus 1
Herpesvirus 1, Human - physiology
Human cytomegalovirus
Human herpesvirus 1
Human herpesvirus 5
Humans
Immediate-early proteins
Intrinsic antiviral defenses
Nuclear Proteins - metabolism
Osteosarcoma
Osteosarcoma - metabolism
Osteosarcoma - virology
Osteosarcoma cells
Phosphoproteins
Plasmids
pp71
Replication
U2OS cells
Viral Proteins - genetics
Viral Proteins - metabolism
virion
Virion - genetics
Virion - physiology
Virions
Virus Replication
X-linked Nuclear Protein
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The control of human cytomegalovirus (HCMV) immediate early (IE) gene expression in infected human fibroblasts was compared with that in the U2OS human osteosarcoma cells. Viral IE expression was stimulated by the virion protein pp71 and repressed by the cell protein hDaxx in fibroblasts, as expected from published data. Neither of these events occurred in infected U2OS cells, suggesting that this cell line lacks one or more factors that repress HCMV IE expression. The chromatin remodeling factor ATRX is absent from U2OS cells, therefore the effect of introducing this protein by electroporation of plasmid DNA was investigated. Provision of ATRX inhibited HCMV IE expression, and the presence of the HCMV-specified virion phosphoprotein pp71 overcame the repression. The experiments demonstrate that ATRX can act as a cellular intrinsic antiviral defense in U2OS cells by blocking gene expression from incoming HCMV genomes. In contrast, ATRX did not affect the replication of herpes simplex virus type 1, showing that there are differences in the way U2OS cells respond to the presence of the herpesviral genomes.
ISSN:0168-1702
1872-7492
DOI:10.1016/j.virusres.2011.02.002