novel multiplex PCR assay for the identification of Indian crocodiles

Illegal hunting has been a major threat for the survival of wildlife fauna, including the three crocodile species that India harbours: Crocodylus palustris, Crocodylus porosus and Gavialis gangeticus. Although law prevents trade on these species, illicit hunting for trade continues to threaten the s...

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Bibliographic Details
Published in:Molecular ecology resources 2010-07, Vol.10 (4), p.744-747
Main Authors: MEGANATHAN, P.R, DUBEY, BHAWNA, JOGAYYA, KOTA NAGA, WHITAKER, NIKHIL, HAQUE, IKRAMUL
Format: Article
Language:English
Subjects:
Aquatic reptiles
Assaying
conservation
Crocodiles
Crocodylia
Crocodylus palustris
Crocodylus porosus
cytochrome b
Electrophoresis
Endangered species
Gavialis gangeticus
Gel electrophoresis
Hunting
Indian crocodiles
molecular identification
multiplex PCR
Multiplexing
Polymerase chain reaction
species-specific primers
Survival
Wildlife
Wildlife conservation
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Summary:Illegal hunting has been a major threat for the survival of wildlife fauna, including the three crocodile species that India harbours: Crocodylus palustris, Crocodylus porosus and Gavialis gangeticus. Although law prevents trade on these species, illicit hunting for trade continues to threaten the survival of these endangered species; conservation strategies therefore require a rapid molecular identification technique for Indian crocodiles. A multiplex polymerase chain reaction (PCR) assay with species-specific primers, considered as one of the most effective molecular techniques, is described herein. The primers were designed to yield species-specific sized amplicons. The assay discriminates the three Indian crocodile species unambiguously within a short time period using only simple agarose gel electrophoresis. We recommend this multiplex PCR assay to be used in the identification of Indian crocodile species.
ISSN:1755-0998
1755-098X
1755-0998
DOI:10.1111/j.1755-0998.2010.02834.x