Rejuvenation improves roller pump-induced physical stress resistance of fresh and stored red blood cells

BACKGROUND: Retrospective studies on transfusion recipients suggested that transfusion of older red blood cells (RBCs) was associated with higher morbidity. Similar studies were also done on cardiac surgery patients who were placed on cardiac bypass pumps. It is possible that stored RBCs are more fr...

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Bibliographic Details
Published in:Transfusion (Philadelphia, Pa.) Pa.), 2011-05, Vol.51 (5), p.1096-1104
Main Authors: Gelderman, Monique P., Vostal, Jaroslav G.
Format: Article
Language:English
Subjects:
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
Animals
Biological and medical sciences
Blood Preservation - methods
Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis
Bone marrow, stem cells transplantation. Graft versus host reaction
Cardiopulmonary Bypass - adverse effects
Erythrocytes - cytology
Erythrocytes - physiology
Humans
Hypotonic Solutions
Infusion Pumps - adverse effects
Medical sciences
Mice
Mice, SCID
Models, Animal
Osmotic Fragility
Osmotic Pressure - physiology
Rejuvenation - physiology
Sodium Chloride
Stress, Mechanical
Transfusions. Complications. Transfusion reactions. Cell and gene therapy
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Summary:BACKGROUND: Retrospective studies on transfusion recipients suggested that transfusion of older red blood cells (RBCs) was associated with higher morbidity. Similar studies were also done on cardiac surgery patients who were placed on cardiac bypass pumps. It is possible that stored RBCs are more fragile and could be more easily damaged by these pumps, thus leading to additional morbidity. STUDY DESIGN AND METHODS: Fresh and stored (42 days) RBCs, rejuvenated and nonrejuvenated, were compared in resistance to physical stress, induced by a roller pump, and osmotic fragility changes during physical stress to model RBCs going through cardiac bypass instruments. In addition, posttransfusion in vivo recovery was evaluated in an immunodeficient mouse model to minimize species differences between transfusion product and recipient. RESULTS: Fresh RBCs were more resistant to both osmotic and physical stress than stored cells. After 2 hours of physical stress, the osmotic stress resistance of fresh cells declined and was the same as for stored cells. Rejuvenated fresh cells did not demonstrate a decline in osmotic resistance during the stress test and both fresh and stored cells had the same improved resistance to osmotic stress before and after the physical stress. Rejuvenation slightly improved recovery of fresh RBCs but almost doubled the recovery of stored cells in the mouse model. CONCLUSIONS: Our studies suggest that rejuvenation improves roller pump–induced physical and osmotic stress resistance of stored RBCs.
ISSN:0041-1132
1537-2995
DOI:10.1111/j.1537-2995.2010.02972.x