Two distinct chimeric potexviruses share antigenic cross-presentation properties of MHC class I epitopes

Abstract Chimeric VLPs made of papaya mosaic virus (PapMV) trigger a CTL response through antigenic presentation of epitopes on MHC class I. Here, a chimeric VLP composed of malva mosaic virus (MaMV) was shown to share similar properties. We demonstrated the capacity of both VLPs to enter human APCs...

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Bibliographic Details
Published in:Vaccine 2010-08, Vol.28 (34), p.5617-5626
Main Authors: Hanafi, Laïla-Aïcha, Bolduc, Marilène, Gagné, Marie-Ève Laliberté, Dufour, Florent, Langelier, Yves, Boulassel, Mohammed-Rachid, Routy, Jean-Pierre, Leclerc, Denis, Lapointe, Réjean
Format: Article
Language:English
Subjects:
Allergy and Immunology
Amino Acid Sequence
Antigen Presentation
Applied microbiology
B-Lymphocytes - immunology
Biological and medical sciences
Capsid Proteins - immunology
CD40 Antigens - immunology
Cell Proliferation
Cloning, Molecular
Cross-presentation
Cross-Priming
Epitopes - immunology
Fundamental and applied biological sciences. Psychology
Human papillomavirus
Humans
Immune system
Malva
Medical research
Microbiology
Miscellaneous
Molecular Sequence Data
Papaya mosaic virus
Phytopathology. Animal pests. Plant and forest protection
Plant viruses and viroids
Potexvirus
Potexvirus - immunology
Protein Engineering
T-Lymphocytes, Cytotoxic - immunology
Vaccines
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
Virology
Virus-like particles
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Summary:Abstract Chimeric VLPs made of papaya mosaic virus (PapMV) trigger a CTL response through antigenic presentation of epitopes on MHC class I. Here, a chimeric VLP composed of malva mosaic virus (MaMV) was shown to share similar properties. We demonstrated the capacity of both VLPs to enter human APCs. The chimeric constructions were cross-presented in CD40-activated B lymphocytes leading to in vitro expansion of antigen-specific T lymphocytes. We showed that high concentrations of chimeric MaMV induced cell death, suggesting that some modifications can trigger collateral effects in vitro . Results suggest that potexvirus VLPs are an attractive vaccine platform for inducing a CTL response.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2010.06.024