Hydrogen-Rich Saline Protects Against Renal Ischemia/Reperfusion Injury in Rats

Background Recently it has been demonstrated that hydrogen, as a novel antioxidant, can selectively reduce hydroxyl radicals (·OH) and peroxynitrite anion (ONOO-) in vitro and exert therapeutic antioxidant activity in many diseases. This study was designed to investigate the effect of hydrogen-rich...

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Published in:The Journal of surgical research 2011-05, Vol.167 (2), p.e339-e344
Main Authors: Wang, Fei, M.D, Yu, Guang, M.D, Liu, Sui-Yi, M.D, Li, Jin-Bao, M.D, Wang, Jia-Feng, M.D, Bo, Lu-Long, M.D, Qian, Li-Ren, M.D, Sun, Xue-Jun, Ph.D, Deng, Xiao-Ming, M.D
Format: Article
Language:English
Subjects:
Animals
antioxidant
Cytokines - metabolism
Deoxyguanosine - analogs & derivatives
Deoxyguanosine - metabolism
Disease Models, Animal
hydrogen
Hydrogen - therapeutic use
ischemia/reperfusion
Kidney - blood supply
Kidney - metabolism
Kidney - physiopathology
Male
Malondialdehyde - metabolism
Neutrophil Infiltration - drug effects
Neutrophil Infiltration - physiology
oxidative stress
Oxidative Stress - drug effects
Oxidative Stress - physiology
Rats
Rats, Sprague-Dawley
Reperfusion Injury - physiopathology
Reperfusion Injury - prevention & control
Sodium Chloride - therapeutic use
Surgery
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Summary:Background Recently it has been demonstrated that hydrogen, as a novel antioxidant, can selectively reduce hydroxyl radicals (·OH) and peroxynitrite anion (ONOO-) in vitro and exert therapeutic antioxidant activity in many diseases. This study was designed to investigate the effect of hydrogen-rich saline on renal ischemia/reperfusion (I/R) injury in rats. Methods A rat model of renal I/R injury was induced by 45-min occlusion of the bilateral renal pedicles and 24-h reperfusion. Physiologic saline, hydrogen-rich saline, or nitrogen-rich saline (8 mL/kg) were administered intraperitoneally at 5 min before reperfusion, respectively. Results After I/R injury, serum blood urea nitrogen (BUN), creatinine (Cr), tissue malondialdehyde (MDA), 8-hydroxydeoxyguanosine (8-OhdG), TNF-α, IL-1β, IL-6 levels, and myeloperoxidase (MPO) activity were all increased significantly, while tissue superoxide dismutase (SOD) and catalase (CAT) activities were all decreased significantly. Hydrogen-rich saline reversed these changes and relieved morphological renal injury and I/R-induced apoptosis, while no significant changes were observed in the nitrogen-rich saline-treated group compared with physiologic saline-treated group. Conclusions Hydrogen-rich saline is able to attenuate the renal I/R injury, which is possibly by reduction of oxidative stress and inflammation.
ISSN:0022-4804
1095-8673
DOI:10.1016/j.jss.2010.11.005