ABA controls H₂O₂ accumulation through the induction of OsCATB in rice leaves under water stress

The production of both ABA and H₂O₂ is induced by drought and can act as signals under stress conditions. We investigated the relationships between ABA, H₂O₂ and catalase (CAT) in rice leaves when rice seedlings were treated with polyethylene glycol as water stress treatment. As a key gene in ABA bi...

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Bibliographic Details
Published in:Plant and cell physiology 2011-04, Vol.52 (4), p.689-698
Main Authors: Ye, Nenghui, Zhu, Guohui, Liu, Yinggao, Li, Yingxuan, Zhang, Jianhua
Format: Article
Language:English
Subjects:
Abscisic Acid - metabolism
Abscisic Acid - pharmacology
Catalase - genetics
Catalase - metabolism
Dehydration - genetics
Dehydration - metabolism
Dioxygenases - genetics
Enzyme Induction - drug effects
Enzyme Inhibitors - pharmacology
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Plant
Hydrogen Peroxide - metabolism
Masoprocol - pharmacology
Oryza - drug effects
Oryza - enzymology
Oryza - genetics
Oryza - metabolism
Phylogeny
Plant Leaves - drug effects
Plant Leaves - enzymology
Plant Leaves - genetics
Plant Leaves - metabolism
Plant Proteins - genetics
Polyethylene Glycols - pharmacology
Reactive Oxygen Species
Reverse Transcriptase Polymerase Chain Reaction
Seedlings - enzymology
Seedlings - genetics
Seedlings - metabolism
Signal Transduction
Tungsten Compounds - pharmacology
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Summary:The production of both ABA and H₂O₂ is induced by drought and can act as signals under stress conditions. We investigated the relationships between ABA, H₂O₂ and catalase (CAT) in rice leaves when rice seedlings were treated with polyethylene glycol as water stress treatment. As a key gene in ABA biosynthesis, OsNCED3 was significantly induced in rice by water stress treatment and such induction preceded the rapid increase in ABA. Water stress inhibited the expression of CATA and CATC but substantially enhanced the expression of CATB. Exogenously applied ABA promoted the expression of CATB also and inhibited the expression of CATC in a concentration-dependent manner. When ABA production was inhibited by using ABA biosynthesis inhibitors nordihydroguaiaretic acid and tungstate, expression of CATB was also subdued while CATC was enhanced under the water stress. Accumulation of H₂O₂ was also reduced when endogenous ABA production was inhibited and showed a correlation with the total activity of catalases. Our results suggest that water stress-induced ABA prevents the excessive accumulation of H₂O₂, through the induction of the expression of CATB gene during water stress.
ISSN:0032-0781
1471-9053
DOI:10.1093/pcp/pcr028