Impedimetric immunosensor based on SWCNT-COOH modified gold microelectrodes for label-free detection of deep venous thrombosis biomarker

Measurement of D-dimer has subsequently become an essential element in the diagnostics of deep vein thrombosis and pulmonary embolism; in this context microelectrodes with an area of 9 × 10 −4 cm 2 were used to develop impedimetric immunosensor for detecting deep venous thrombosis biomarker (D-dimer...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2010-12, Vol.26 (4), p.1278-1282
Main Authors: Bourigua, S., Hnaien, M., Bessueille, F., Lagarde, F., Dzyadevych, S., Maaref, A., Bausells, J., Errachid, A., Renault, N. Jaffrezic
Format: Article
Language:English
Subjects:
Atomic beam spectroscopy
Binding
Biological and medical sciences
Biomarkers - blood
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Biosensors
Biotechnology
Blood Chemical Analysis - instrumentation
Blood Chemical Analysis - methods
Carbon nanotubes
Carbon nanotubes (SWNCT-COOH)
D-dimer
Dielectric Spectroscopy
Electrochemical impedance spectroscopy
Equipment Design
Fibrin Fibrinogen Degradation Products - analysis
Fundamental and applied biological sciences. Psychology
Gold
Humans
Immunosensors
Methods. Procedures. Technologies
Microelectrodes
Microscopy, Atomic Force
Nanotubes, Carbon
Thrombosis
Various methods and equipments
Veins
Venous Thrombosis - blood
Venous Thrombosis - diagnosis
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Summary:Measurement of D-dimer has subsequently become an essential element in the diagnostics of deep vein thrombosis and pulmonary embolism; in this context microelectrodes with an area of 9 × 10 −4 cm 2 were used to develop impedimetric immunosensor for detecting deep venous thrombosis biomarker (D-dimer). The biosensor is based on functionalized carbon nanotubes (SWCNT-COOH) where the antibody (anti-D-dimer) was immobilized by covalent binding. The electrical properties and the morphology of the biolayer were characterized by electrochemical impedance spectroscopy (EIS), cyclic voltammetry and atomic force spectroscopy (AFM). Impedimetric microimmunosensor allows to obtain sensitivity of 40.1 kΩ μM −1 and detection limit of 0.1 pg/mL (0.53 fM) with linear range from 0.1 pg/mL to 2 μg/mL (0.53 fM to 0.01 μM). We demonstrate that using carbon nanotubes and microelectrodes, high sensitivity and dynamic range were obtained. The biosensor exhibited a short response time of 10 min. Moreover, the studied immunosensor exhibits good reproducibility (R.S.D. 8.2%, n = 4).
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2010.07.004