Self―organizing optic―cup morphogenesis in three―dimensional culture

Balanced organogenesis requires the orchestration of multiple cellular interactions to create the collective cell behaviours that progressively shape developing tissues. It is currently unclear how individual, localized parts are able to coordinate with each other to develop a whole organ shape. Her...

Full description

Saved in:

Bibliographic Details
Published in:	Nature (London) 2011-04, Vol.472 (7341), p.51-56
Main Authors:	EIRAKU, Mototsugu, TAKATA, Nozomu, ISHIBASHI, Hiroki, KAWADA, Masako, SAKAKURA, Eriko, OKUDA, Satoru, SEKIGUCHI, Kiyotoshi, ADACHI, Taiji, SASAI, Yoshiki
Format:	Article
Language:	eng
Subjects:	Animals Biological and medical sciences Cell Culture Techniques - methods Cell migration Computer simulation Embryonic development Embryonic stem cells Embryonic Stem Cells - cytology Eye and associated structures. Visual pathways and centers. Vision Fundamental and applied biological sciences. Psychology Mice Morphogenesis Neural Plate - cytology Neural Plate - embryology Neural Stem Cells - cytology Organ Culture Techniques - methods Organogenesis Physiological aspects Regenerative Medicine - methods Retina Retina - cytology Retina - embryology Retinal Pigment Epithelium - cytology Retinal Pigment Epithelium - embryology Vertebrates: nervous system and sense organs
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Balanced organogenesis requires the orchestration of multiple cellular interactions to create the collective cell behaviours that progressively shape developing tissues. It is currently unclear how individual, localized parts are able to coordinate with each other to develop a whole organ shape. Here we report the dynamic, autonomous formation of the optic cup (retinal primordium) structure from a three-dimensional culture of mouse embryonic stem cell aggregates. Embryonic-stem-cell-derived retinal epithelium spontaneously formed hemispherical epithelial vesicles that became patterned along their proximal-distal axis. Whereas the proximal portion differentiated into mechanically rigid pigment epithelium, the flexible distal portion progressively folded inward to form a shape reminiscent of the embryonic optic cup, exhibited interkinetic nuclear migration and generated stratified neural retinal tissue, as seen in vivo. We demonstrate that optic-cup morphogenesis in this simple cell culture depends on an intrinsic self-organizing program involving stepwise and domain-specific regulation of local epithelial properties.
ISSN:	0028-0836 1476-4687